2J07

Thermus DNA photolyase with 8-HDF antenna chromophore

Summary for 2J07

• Entry DOI: 10.2210/pdb2j07/pdb
• Related: 1IQR 1IQU 2CGB 2CGC 2J08 2J09
• Descriptor: DEOXYRIBODIPYRIMIDINE PHOTO-LYASE, FLAVIN-ADENINE DINUCLEOTIDE, 8-HYDROXY-10-(D-RIBO-2,3,4,5-TETRAHYDROXYPENTYL)-5-DEAZAISOALLOXAZINE, ... (6 entities in total)
• Functional Keywords: lyase, flavoprotein, nucleotide-binding, dna repair
• Biological source: THERMUS THERMOPHILUS
• Total number of polymer chains: 1
• Total formula weight: 49324.14

Authors

Klar, T.,Kaiser, G.,Hennecke, U.,Carell, T.,Batschauer, A.,Essen, L.-O. (deposition date: 2006-08-01, release date: 2007-05-01, Last modification date: 2023-12-13)

Primary citation

Klar, T.,Kaiser, G.,Hennecke, U.,Carell, T.,Batschauer, A.,Essen, L.-O.
Natural and Non-Natural Antenna Chromophores in the DNA Photolyase from Thermus Thermophilus
Chembiochem, 7:1798-, 2006

PubMed Abstract: X-ray crystallographic and functional analysis of the class I DNA photolyase from Thermus thermophilus revealed the binding of flavin mononucleotide (FMN) as an antenna chromophore. The binding mode of FMN closely coincides with the binding of a deazaflavin-like chromophore in the related class I DNA photolyase from Anacystis nidulans. Compared to the R46E mutant, which lacks a conserved arginine in the binding site for the antenna chromophore, the FMN-comprising holophotolyase exhibits an eightfold higher activity at 450 nm. The facile incorporation of the flavin cofactors 8-hydroxy-deazariboflavin and 8-iodo-8-demethyl-riboflavin into the binding site for the antenna chromophore paves the way for wavelength-tuning of the activity spectra of DNA photolyases by using synthetic flavins.

PubMed: 17051659
DOI: 10.1002/CBIC.200600206

Experimental method

X-RAY DIFFRACTION (1.95 Å)