2IYA

The crystal structure of macrolide glycosyltransferases: A blueprint for antibiotic engineering

Summary for 2IYA

DescriptorOLEANDOMYCIN GLYCOSYLTRANSFERASE, URIDINE-5'-DIPHOSPHATE, (3S,5R,6S,7R,8R,11R,12S,13R,14S,15S)-6-HYDROXY-5,7,8,11,13,15-HEXAMETHYL-4,10-DIOXO-14-{[3,4,6-TRIDEOXY-3-(DIMETHYLAMINO)-BETA-D-XYLO-HEXOPYRANOSYL]OXY}-1,9-DIOXASPIRO[2.13]HEXADEC-12-YL 2,6-DIDEOXY-3-O-METHYL-ALPHA-L-ARABINO-HEXOPYRANOSIDE, ... (4 entities in total)
Functional Keywordscarbohydrate, glycosylation, glycosyltransferase, enzyme, macrolide, transferase
Biological sourceSTREPTOMYCES ANTIBIOTICUS
Total number of polymer chains2
Total molecular weight93609.76
Authors
Bolam, D.N.,Roberts, S.M.,Proctor, M.R.,Turkenburg, J.P.,Dodson, E.J.,Martinez-Fleites, C.,Yang, M.,Davis, B.G.,Davies, G.J.,Gilbert, H.J. (deposition date: 2006-07-13, release date: 2007-03-27, Last modification date: 2018-01-24)
Primary citation
Bolam, D.N.,Roberts, S.M.,Proctor, M.R.,Turkenburg, J.P.,Dodson, E.J.,Martinez-Fleites, C.,Yang, M.,Davis, B.G.,Davies, G.J.,Gilbert, H.J.
The Crystal Structure of Two Macrolide Glycosyltransferases Provides a Blueprint for Host Cell Antibiotic Immunity.
Proc.Natl.Acad.Sci.USA, 104:5336-, 2007
PubMed: 17376874 (PDB entries with the same primary citation)
DOI: 10.1073/PNAS.0607897104
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.7 Å)
?

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.198201.0%2.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 2iya
no rotation
Molmil generated image of 2iya
rotated about x axis by 90°
Molmil generated image of 2iya
rotated about y axis by 90°

More Biological unit images

Molmil generated image of 2iya
no rotation
Molmil generated image of 2iya
rotated about x axis by 90°
Molmil generated image of 2iya
rotated about y axis by 90°
(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (2iya.pdb1.gz [69.96 KB])
Coordinate files for Biological unit (2iya.pdb2.gz [68.94 KB])