2ISL

BluB bound to reduced flavin (FMNH2) and molecular oxygen. (clear crystal form)

Summary for 2ISL

• Entry DOI: 10.2210/pdb2isl/pdb
• Related: 2ISJ 2ISK
• Descriptor: BluB, 1-DEOXY-1-(7,8-DIMETHYL-2,4-DIOXO-3,4-DIHYDRO-2H-BENZO[G]PTERIDIN-1-ID-10(5H)-YL)-5-O-PHOSPHONATO-D-RIBITOL, OXYGEN MOLECULE, ... (4 entities in total)
• Functional Keywords: flavin, oxidoreductase, monooxygenase, flavin destructase, molecular oxygen, flavoprotein
• Biological source: Sinorhizobium meliloti
• Total number of polymer chains: 8
• Total formula weight: 210485.82

Authors

Larsen, N.A.,Taga, M.E.,Howard-Jones, A.R.,Walsh, C.T.,Walker, G.C. (deposition date: 2006-10-17, release date: 2007-03-27, Last modification date: 2024-02-21)

Primary citation

Taga, M.E.,Larsen, N.A.,Howard-Jones, A.R.,Walsh, C.T.,Walker, G.C.
BluB cannibalizes flavin to form the lower ligand of vitamin B12.
Nature, 446:449-453, 2007

PubMed Abstract: Vitamin B12 (cobalamin) is among the largest known non-polymeric natural products, and the only vitamin synthesized exclusively by microorganisms. The biosynthesis of the lower ligand of vitamin B(12), 5,6-dimethylbenzimidazole (DMB), is poorly understood. Recently, we discovered that a Sinorhizobium meliloti gene, bluB, is necessary for DMB biosynthesis. Here we show that BluB triggers the unprecedented fragmentation and contraction of the bound flavin mononucleotide cofactor and cleavage of the ribityl tail to form DMB and D-erythrose 4-phosphate. Our structural analysis shows that BluB resembles an NAD(P)H-flavin oxidoreductase, except that its unusually tight binding pocket accommodates flavin mononucleotide but not NAD(P)H. We characterize crystallographically an early intermediate along the reaction coordinate, revealing molecular oxygen poised over reduced flavin. Thus, BluB isolates and directs reduced flavin to activate molecular oxygen for its own cannibalization. This investigation of the biosynthesis of DMB provides clarification of an aspect of vitamin B12 that was otherwise incomplete, and may contribute to a better understanding of vitamin B12-related disease.

PubMed: 17377583
DOI: 10.1038/nature05611

Experimental method

X-RAY DIFFRACTION (2.9 Å)