2IMP

Crystal structure of lactaldehyde dehydrogenase from E. coli: the ternary complex with lactate (occupancy 0.5) and NADH. Crystals soaked with (L)-Lactate.

2IMP の概要

• エントリーDOI: 10.2210/pdb2imp/pdb
• 関連するPDBエントリー: 2HG2 2ILU
• 分子名称: Lactaldehyde dehydrogenase, SULFATE ION, 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE, ... (5 entities in total)
• 機能のキーワード: protein-lactate-nadh ternary complex, oxidoreductase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 53320.17

構造登録者

Di Costanzo, L.,Gomez, G.A.,Christianson, D.W. (登録日: 2006-10-04, 公開日: 2007-02-13, 最終更新日: 2023-11-15)

主引用文献

Di Costanzo, L.,Gomez, G.A.,Christianson, D.W.
Crystal Structure of Lactaldehyde Dehydrogenase from Escherichia coli and Inferences Regarding Substrate and Cofactor Specificity.
J.Mol.Biol., 366:481-493, 2007

PubMed Abstract: Aldehyde dehydrogenases catalyze the oxidation of aldehyde substrates to the corresponding carboxylic acids. Lactaldehyde dehydrogenase from Escherichia coli (aldA gene product, P25553) is an NAD(+)-dependent enzyme implicated in the metabolism of l-fucose and l-rhamnose. During the heterologous expression and purification of taxadiene synthase from the Pacific yew, lactaldehyde dehydrogenase from E. coli was identified as a minor (PubMed: 17173928
DOI: 10.1016/j.jmb.2006.11.023

実験手法

X-RAY DIFFRACTION (2.1 Å)