Loading
PDBj
メニューPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

2IL4

Crystal structure of At1g77540-Coenzyme A Complex

2GDB」から置き換えられました
2IL4 の概要
エントリーDOI10.2210/pdb2il4/pdb
関連するPDBエントリー1xmt 2evn
分子名称Protein At1g77540, COENZYME A (3 entities in total)
機能のキーワードcoa, coenzyme-a, cog2388 family, acetyltransferase, at1g77540, structural genomics functional follow-up study, protein structure initiative, psi, center for eukaryotic structural genomics, cesg, transferase
由来する生物種Arabidopsis thaliana (thale cress)
タンパク質・核酸の鎖数1
化学式量合計12519.97
構造登録者
Bitto, E.,Wesenberg, G.E.,Phillips Jr., G.N.,Bingman, C.A.,Center for Eukaryotic Structural Genomics (CESG) (登録日: 2006-10-02, 公開日: 2006-10-17, 最終更新日: 2023-08-30)
主引用文献Tyler, R.C.,Bitto, E.,Berndsen, C.E.,Bingman, C.A.,Singh, S.,Lee, M.S.,Wesenberg, G.E.,Denu, J.M.,Phillips, G.N.,Markley, J.L.
Structure of Arabidopsis thaliana At1g77540 Protein, a Minimal Acetyltransferase from the COG2388 Family.
Biochemistry, 45:14325-14336, 2006
Cited by
PubMed Abstract: We describe X-ray crystal and NMR solution structures of the protein coded for by Arabidopsis thaliana gene At1g77540.1 (At1g77540). The crystal structure was determined to 1.15 A with an R factor of 14.9% (Rfree = 17.0%) by multiple-wavelength anomalous diffraction using sodium bromide derivatized crystals. The ensemble of NMR conformers was determined with protein samples labeled with 15N and 13C + 15N. The X-ray structure and NMR ensemble were closely similar with rmsd 1.4 A for residues 8-93. At1g77540 was found to adopt a fold similar to that of GCN5-related N-acetyltransferases. Enzymatic activity assays established that At1g77540 possesses weak acetyltransferase activity against histones H3 and H4. Chemical shift perturbations observed in 15N-HSQC spectra upon the addition of CoA indicated that the cofactor binds and identified its binding site. The molecular details of this interaction were further elucidated by solving the X-ray structure of the At1g77540-CoA complex. This work establishes that the domain family COG2388 represents a novel class of acetyltransferase and provides insight into possible mechanistic roles of the conserved Cys76 and His41 residues of this family.
PubMed: 17128971
DOI: 10.1021/bi0612059
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.054 Å)
構造検証レポート
Validation report summary of 2il4
検証レポート(詳細版)ダウンロードをダウンロード

234136

件を2025-04-02に公開中

PDB statisticsPDBj update infoContact PDBjnumon