2IJX

Crystal structure of PCNA3 monomer from Sulfolobus solfataricus.

2IJX の概要

• エントリーDOI: 10.2210/pdb2ijx/pdb
• 分子名称: DNA polymerase sliding clamp A, 1,2-ETHANEDIOL (3 entities in total)
• 機能のキーワード: pcna3 subunit, protein-protein interaction, pcna123 heterotrimer, dna binding protein
• 由来する生物種: Sulfolobus solfataricus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 111197.84

構造登録者

Hlinkova, V.,Ling, H. (登録日: 2006-10-01, 公開日: 2007-10-16, 最終更新日: 2023-08-30)

主引用文献

Hlinkova, V.,Xing, G.,Bauer, J.,Shin, Y.J.,Dionne, I.,Rajashankar, K.R.,Bell, S.D.,Ling, H.
Structures of monomeric, dimeric and trimeric PCNA: PCNA-ring assembly and opening.
Acta Crystallogr.,Sect.D, 64:941-949, 2008

PubMed Abstract: DNA sliding clamps form an oligomeric ring encircling DNA and serve as a moving platform for DNA-processing proteins. The opening and closing of a sliding-clamp ring is essential to load the clamp onto DNA in order to perform its functions. The molecular details of how clamp rings open and enclose DNA are still not clear. Three PCNA homologues have been found in Sulfolobus solfataricus which form a heterotrimer. Taking advantage of their hetero-oligomeric nature, the structures of the PCNAs in monomeric PCNA3, dimeric PCNA1-PCNA2 and trimeric PCNA1-PCNA2-PCNA3 forms were determined at resolutions of 2.6-1.9 A. The distinct oligomeric structures represent different stages in ring formation, which were verified in solution by ultracentrifugation analysis. The heterodimer opens in a V-shape of 130 degrees , while the heterotrimers form a ring with a 120 degrees rotation between monomers. The association of a rigid PCNA3 monomer with an opened PCNA1-PCNA2 heterodimer closes the ring and introduces a spring tension in the PCNA1-PCNA2 interface, thus bending the nine-stranded intermolecular beta-sheet to fit the 120 degrees rotation. The release of the spring tension as PCNA3 dissociates from the ring may facilitate ring opening. The structural features in different assemblies present a molecular model for clamp ring assembly and opening.

PubMed: 18703842
DOI: 10.1107/S0907444908021665

実験手法

X-RAY DIFFRACTION (1.9 Å)