2ID0

Escherichia coli RNase II

2ID0 の概要

• エントリーDOI: 10.2210/pdb2id0/pdb
• 分子名称: Exoribonuclease 2, MANGANESE (II) ION (3 entities in total)
• 機能のキーワード: rnase, exoribonuclease, ribonuclease, exonuclease, nuclease, hydrolyase, mrna decay, rnr family, hydrolase
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: P30850
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 293388.41

構造登録者

Zuo, Y.,Zhang, J.,Wang, Y.,Malhotra, A. (登録日: 2006-09-13, 公開日: 2006-10-03, 最終更新日: 2024-11-13)

主引用文献

Zuo, Y.,Vincent, H.A.,Zhang, J.,Wang, Y.,Deutscher, M.P.,Malhotra, A.
Structural Basis for Processivity and Single-Strand Specificity of RNase II.
Mol.Cell, 24:149-156, 2006

PubMed Abstract: RNase II is a member of the widely distributed RNR family of exoribonucleases, which are highly processive 3'-->5' hydrolytic enzymes that play an important role in mRNA decay. Here, we report the crystal structure of E. coli RNase II, which reveals an architecture reminiscent of the RNA exosome. Three RNA-binding domains come together to form a clamp-like assembly, which can only accommodate single-stranded RNA. This leads into a narrow, basic channel that ends at the putative catalytic center that is completely enclosed within the body of the protein. The putative path for RNA agrees well with biochemical data indicating that a 3' single strand overhang of 7-10 nt is necessary for binding and hydrolysis by RNase II. The presence of the clamp and the narrow channel provides an explanation for the processivity of RNase II and for why its action is limited to single-stranded RNA.

PubMed: 16996291
DOI: 10.1016/j.molcel.2006.09.004

実験手法

X-RAY DIFFRACTION (2.35 Å)