2IBZ

Yeast Cytochrome BC1 Complex with Stigmatellin

2IBZ の概要

• エントリーDOI: 10.2210/pdb2ibz/pdb
• 関連するPDBエントリー: 1EZV 1KB9 1KYO 1P84
• 分子名称: Ubiquinol-cytochrome-c reductase complex core protein 1, Variable Heavy chain of antibody fragment, Variable Light chain of antibody fragment, ... (16 entities in total)
• 機能のキーワード: multisubunit membrane protein complex, oxidoreductase
• 由来する生物種: Mus musculus (house mouse); 詳細
• 細胞内の位置: Mitochondrion inner membrane : P07256 P07257 P00127 P00128 P08525 P22289; Mitochondrion inner membrane ; Multi-pass membrane protein : P00163; Mitochondrion inner membrane; Single-pass membrane protein; Intermembrane side: P07143; Mitochondrion inner membrane ; Single-pass membrane protein : P08067
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 249144.47

構造登録者

Hunte, C. (登録日: 2006-09-12, 公開日: 2007-03-20, 最終更新日: 2024-11-13)

主引用文献

Lancaster, C.R.,Hunte, C.,Kelley, J.,Trumpower, B.L.,Ditchfield, R.
A Comparison of Stigmatellin Conformations, Free and Bound to the Photosynthetic Reaction Center and the Cytochrome bc(1) Complex.
J.Mol.Biol., 368:197-208, 2007

PubMed Abstract: We describe in detail the conformations of the inhibitor stigmatellin in its free form and bound to the ubiquinone-reducing (Q(B)) site of the reaction center and to the ubiquinol-oxidizing (Q(o)) site of the cytochrome bc(1) complex. We present here the first structures of a stereochemically correct stigmatellin in complexes with a bacterial reaction center and the yeast cytochrome bc1 complex. The conformations of the inhibitor bound to the two enzymes are not the same. We focus on the orientations of the stigmatellin side-chain relative to the chromone head group, and on the interaction of the stigmatellin side-chain with these membrane protein complexes. The different conformations of stigmatellin found illustrate the structural variability of the Q sites, which are affected by the same inhibitor. The free rotation about the chi1 dihedral angle is an essential factor for allowing stigmatellin to bind in both the reaction center and the cytochrome bc1 pocket.

PubMed: 17337272
DOI: 10.1016/j.jmb.2007.02.013

実験手法

X-RAY DIFFRACTION (2.3 Å)