2IBM
A novel dimer interface and conformational changes revealed by an X-ray structure of B. subtilis SecA
Summary for 2IBM
| Entry DOI | 10.2210/pdb2ibm/pdb |
| Related | 1M6N 1NKT 1TF2 |
| Descriptor | Preprotein translocase secA subunit, ADENOSINE-5'-DIPHOSPHATE (2 entities in total) |
| Functional Keywords | protein translocation, seca, signal peptide binding, protein transport |
| Biological source | Bacillus subtilis |
| Cellular location | Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): P28366 |
| Total number of polymer chains | 2 |
| Total formula weight | 178259.56 |
| Authors | Zimmer, J.,Li, W.,Rapoport, T.A. (deposition date: 2006-09-11, release date: 2006-11-14, Last modification date: 2023-08-30) |
| Primary citation | Zimmer, J.,Li, W.,Rapoport, T.A. A Novel Dimer Interface and Conformational Changes Revealed by an X-ray Structure of B. subtilis SecA. J.Mol.Biol., 364:259-265, 2006 Cited by PubMed Abstract: The SecA ATPase moves polypeptides post-translationally across the plasma membrane of eubacteria, but the mechanism of transport is still unclear. We describe the crystal structure of a novel dimeric form of Bacillus subtilis SecA. Dimerization of SecA occurs at the prominent groove formed by the nucleotide binding domain 2 (nbd2) and the preprotein cross-linking (ppx) domain. The dimer interface is very large, burying approximately 5400 A(2) of solvent accessible surface per monomer. Single cysteine disulfide cross-linking shows the presence of this novel SecA dimer in solution. In addition, other dimers also exist in solution, arguing that they all are in equilibrium with monomeric SecA and supporting the idea that the monomer may be the functional species. Dimerization of SecA causes an alpha-helix of one subunit to convert to a short beta-strand that participates in beta-sheet formation with strands in the other subunit. This conversion of secondary structure elements occurs close to the connection between the nbd1 and ppx domains, a potential site of interaction with translocation substrate. Comparing the different X-ray structures of B. subtilis SecA suggests that small changes in the nucleotide binding domains could be amplified via helix 1 of the helical scaffold domain (hsd) to generate larger movements of the domains involved in polypeptide binding. PubMed: 16989859DOI: 10.1016/j.jmb.2006.08.044 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.2 Å) |
Structure validation
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