2I3B

Solution Structure of a Human Cancer-Related Nucleoside Triphosphatase

Summary for 2I3B

• Entry DOI: 10.2210/pdb2i3b/pdb
• NMR Information: BMRB: 7119
• Descriptor: Human Cancer-Related NTPase (1 entity in total)
• Functional Keywords: ntpase, aaa, rossmann, hydrolase
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 20610.87

Authors

Placzek, W.J.,Almeida, M.S.,Wuthrich, K. (deposition date: 2006-08-17, release date: 2007-03-13, Last modification date: 2024-05-29)

Primary citation

Placzek, W.J.,Almeida, M.S.,Wuthrich, K.
NMR Structure and Functional Characterization of a Human Cancer-related Nucleoside Triphosphatase.
J.Mol.Biol., 367:788-801, 2007

PubMed Abstract: A screen of the human cancer genome anatomy project (CGAP) database was performed to search for new proteins involved in tumorigenesis. The resulting hits were further screened for recombinant expression, solubility and protein aggregation, which led to the identification of the previously unknown human cancer-related (HCR) protein encoded by the mRNA NM_032324 as a target for structure determination by NMR. The three-dimensional structure of the protein in its complex with ATPgammaS forms a three-layered alpha/beta sandwich, with a central nine-stranded beta-sheet surrounded by five alpha-helices. Sequence and three-dimensional structure comparisons with AAA+ ATPases revealed the presence of Walker A (GPPGVGKT) and Walker B (VCVIDEIG) motifs. Using 1D (31)P-NMR spectroscopy and a coupled enzymatic assay for the determination of inorganic phosphate, we showed that the purified recombinant protein is active as a non-specific nucleoside triphosphatase, with k(cat)=7.6x10(-3) s(-1). The structural basis for the enzymatic activity of HCR-NTPase was further characterized by site-directed mutagenesis of the Walker B motif, which further contributes to making the HCR-NTPase an attractive new target for further biochemical characterization in the context of its presumed role in human tumorigenesis.

PubMed: 17291528
DOI: 10.1016/j.jmb.2007.01.001

Experimental method

SOLUTION NMR