2HZM

Structure of the Mediator head subcomplex Med18/20

2HZM の概要

• エントリーDOI: 10.2210/pdb2hzm/pdb
• 関連するPDBエントリー: 1YKE 1YKH 1ZP2 2HZS
• 分子名称: RNA polymerase II mediator complex subunit 20, RNA polymerase II mediator complex subunit 18, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: beta barrel, channel, transcription
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Nucleus: P34162 P32585
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 234390.93

構造登録者

Lariviere, L.,Geiger, S.,Hoeppner, S.,Rother, S.,Straesser, K.,Cramer, P. (登録日: 2006-08-09, 公開日: 2006-09-12, 最終更新日: 2024-02-21)

主引用文献

Lariviere, L.,Geiger, S.,Hoeppner, S.,Rother, S.,Strasser, K.,Cramer, P.
Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20.
Nat.Struct.Mol.Biol., 13:895-901, 2006

PubMed Abstract: The Mediator head module stimulates basal RNA polymerase II (Pol II) transcription and enables transcriptional regulation. Here we show that the head subunits Med8, Med18 and Med20 form a subcomplex (Med8/18/20) with two submodules. The highly conserved N-terminal domain of Med8 forms one submodule that binds the TATA box-binding protein (TBP) in vitro and is essential in vivo. The second submodule consists of the C-terminal region of Med8 (Med8C), Med18 and Med20. X-ray analysis of this submodule reveals that Med18 and Med20 form related beta-barrel folds. A conserved putative protein-interaction face on the Med8C/18/20 submodule includes sites altered by srb mutations, which counteract defects resulting from Pol II truncation. Our results and published data support a positive role of the Med8/18/20 subcomplex in initiation-complex formation and suggest that the Mediator head contains a multipartite TBP-binding site that can be modulated by transcriptional activators.

PubMed: 16964259
DOI: 10.1038/nsmb1143

実験手法

X-RAY DIFFRACTION (2.4 Å)