2HW8

Structure of ribosomal protein L1-mRNA complex at 2.1 resolution.

2HW8 の概要

• エントリーDOI: 10.2210/pdb2hw8/pdb
• 関連するPDBエントリー: 1U63 1ZHO
• 分子名称: 36-MER, 50S ribosomal protein L1, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: ribosomal protein l1, mrna, rna-protein complex, structural protein-rna complex, structural protein/rna
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36683.74

構造登録者

Tishchenko, S.,Nikonova, E.,Nikulin, A.,Nevskaya, N.,Volchkov, S.,Piendl, W.,Garber, M.,Nikonov, S. (登録日: 2006-08-01, 公開日: 2006-12-19, 最終更新日: 2024-10-09)

主引用文献

Tishchenko, S.,Nikonova, E.,Nikulin, A.,Nevskaya, N.,Volchkov, S.,Piendl, W.,Garber, M.,Nikonov, S.
Structure of the ribosomal protein L1-mRNA complex at 2.1 A resolution: common features of crystal packing of L1-RNA complexes.
ACTA CRYSTALLOGR.,SECT.D, 62:1545-1554, 2006

PubMed Abstract: The crystal structure of a hybrid complex between the bacterial ribosomal protein L1 from Thermus thermophilus and a Methanococcus vannielii mRNA fragment containing an L1-binding site was determined at 2.1 A resolution. It was found that all polar atoms involved in conserved protein-RNA hydrogen bonds have high values of density in the electron-density map and that their hydrogen-bonding capacity is fully realised through interactions with protein atoms, water molecules and K(+) ions. Intermolecular contacts were thoroughly analyzed in the present crystals and in crystals of previously determined L1-RNA complexes. It was shown that extension of the RNA helices providing canonical helix stacking between open-open or open-closed ends of RNA fragments is a common feature of these and all known crystals of complexes between ribosomal proteins and RNAs. In addition, the overwhelming majority of complexes between ribosomal proteins and RNA molecules display crystal contacts formed by the central parts of the RNA fragments. These contacts are often very extensive and strong and it is proposed that they are formed in the saturated solution prior to crystal formation.

PubMed: 17139090
DOI: 10.1107/S0907444906041655

実験手法

X-RAY DIFFRACTION (2.1 Å)