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2HVZ

Solution structure of the RRM domain of SR rich factor 9G8

Summary for 2HVZ
Entry DOI10.2210/pdb2hvz/pdb
NMR InformationBMRB: 7257
DescriptorSplicing factor, arginine/serine-rich 7 (1 entity in total)
Functional Keywordsrrm, rna binding protein
Biological sourceHomo sapiens (human)
Cellular locationNucleus: Q16629
Total number of polymer chains1
Total formula weight11338.99
Authors
Tintaru, A.M.,Hautbergue, G.M.,Golovanov, A.P.,Lian, L.Y.,Wilson, S.A. (deposition date: 2006-07-31, release date: 2006-11-21, Last modification date: 2024-05-29)
Primary citationHargous, Y.,Hautbergue, G.M.,Tintaru, A.M.,Skrisovska, L.,Golovanov, A.P.,Stevenin, J.,Lian, L.Y.,Wilson, S.A.,Allain, F.H.
Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8.
Embo J., 25:5126-5137, 2006
Cited by
PubMed Abstract: The sequence-specific RNA-binding proteins SRp20 and 9G8 are the smallest members of the serine- and arginine-rich (SR) protein family, well known for their role in splicing. They also play a role in mRNA export, in particular of histone mRNAs. We present the solution structures of the free 9G8 and SRp20 RNA recognition motifs (RRMs) and of SRp20 RRM in complex with the RNA sequence 5'CAUC3'. The SRp20-RNA structure reveals that although all 4 nt are contacted by the RRM, only the 5' cytosine is primarily recognized in a specific way. This might explain the numerous consensus sequences found by SELEX (systematic evolution of ligands by exponential enrichment) for the RRM of 9G8 and SRp20. Furthermore, we identify a short arginine-rich peptide adjacent to the SRp20 and 9G8 RRMs, which does not contact RNA but is necessary and sufficient for interaction with the export factor Tip-associated protein (TAP). Together, these results provide a molecular description for mRNA and TAP recognition by SRp20 and 9G8.
PubMed: 17036044
DOI: 10.1038/sj.emboj.7601385
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

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