2HTJ
NMR structure of E.coli PapI
Summary for 2HTJ
| Entry DOI | 10.2210/pdb2htj/pdb |
| Descriptor | P fimbrial regulatory protein KS71A (1 entity in total) |
| Functional Keywords | winged helix-turn-helix, pap pili, transcription activator |
| Biological source | Escherichia coli |
| Total number of polymer chains | 1 |
| Total formula weight | 9338.63 |
| Authors | Kawamura, T.,Zhou, H.,Le, L.U.K.,Dahlquist, F.W. (deposition date: 2006-07-25, release date: 2007-01-30, Last modification date: 2024-05-29) |
| Primary citation | Kawamura, T.,Le, L.U.,Zhou, H.,Dahlquist, F.W. Solution Structure of Escherichia coli PapI, a Key Regulator of the Pap Pili Phase Variation. J.Mol.Biol., 365:1130-1142, 2007 Cited by PubMed Abstract: Pyelonephritis-associated pili (pap) allow uropathogenic Escherichia coli to bind to epithelial cells and play an important role in urinary tract infection. Expression of pap is controlled by a phase-variation mechanism, based on the two distinct heritable states that are the result of adenine N6-methylation in either of the two GATC sequences in its regulatory region. The methylation status of these two sequences is sensed by the action of two proteins, Lrp and PapI, and they play a central role in determining pap gene expression in both phase-ON and phase-OFF cells. We used modern NMR techniques to determine the solution structure and backbone dynamics of PapI. We found its overall fold resembles closely that of the winged helix-turn-helix family of DNA-binding proteins. We determined that PapI possesses its own DNA-binding activity, albeit non-sequence-specific, independent of Lrp. PapI appears to bind to DNA with a K(d) in the 10 microM range. Possible mechanisms by which PapI might participate in the regulation of the pap operon are discussed in light of these new findings. PubMed: 17109885DOI: 10.1016/j.jmb.2006.10.066 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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