2HFC

Structure of S65T Y66F R96A GFP variant in precursor state

Summary for 2HFC

Related2HCG 1QXT
DescriptorGreen fluorescent protein, MAGNESIUM ION (3 entities in total)
Functional Keywordspost-translational modification, cyclization, fluorophore, x-ray damage, luminescent protein
Biological sourceAequorea victoria
Total number of polymer chains1
Total molecular weight26777.36
Authors
Barondeau, D.P.,Kassmann, C.J.,Tainer, J.A.,Getzoff, E.D. (deposition date: 2006-06-23, release date: 2007-03-27, Last modification date: 2011-07-13)
Primary citation
Barondeau, D.P.,Kassmann, C.J.,Tainer, J.A.,Getzoff, E.D.
The Case of the Missing Ring: Radical Cleavage of a Carbon-Carbon Bond and Implications for GFP Chromophore Biosynthesis
J.Am.Chem.Soc., 129:3118-3126, 2007
PubMed: 17326633 (PDB entries with the same primary citation)
DOI: 10.1021/ja063983u
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.2 Å)
NMR Information
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.205304.5%3.9%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 2hfc
no rotation
Molmil generated image of 2hfc
rotated about x axis by 90°
Molmil generated image of 2hfc
rotated about y axis by 90°
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