2HAN

Structural basis of heterodimeric ecdysteroid receptor interaction with natural response element hsp27 gene promoter

Summary for 2HAN

• Entry DOI: 10.2210/pdb2han/pdb
• Descriptor: 5'-D(*CP*AP*AP*GP*GP*GP*TP*TP*CP*AP*AP*TP*GP*CP*AP*CP*TP*TP*GP*T)-3', 5'-D(*GP*AP*CP*AP*AP*GP*TP*GP*CP*AP*TP*TP*GP*AP*AP*CP*CP*CP*TP*T)-3', Protein ultraspiracle, ... (6 entities in total)
• Functional Keywords: transcription regulation, transcription factor, dna-binding, nuclear protein, nuclear receptor, zinc finger, transcription-dna complex, transcription/dna
• Biological source: Drosophila melanogaster (fruit fly); More
• Cellular location: Nucleus: P20153 P34021
• Total number of polymer chains: 4
• Total formula weight: 36867.86

Authors

Jakob, M.,Kolodziejczyk, R.,Orlowski, M.,Krzywda, S.,Kowalska, A.,Dutko-Gwozdz, J.,Gwozdz, T.,Kochman, M.,Jaskolski, M.,Ozyhar, A. (deposition date: 2006-06-13, release date: 2007-05-22, Last modification date: 2023-08-30)

Primary citation

Jakob, M.,Kolodziejczyk, R.,Orlowski, M.,Krzywda, S.,Kowalska, A.,Dutko-Gwozdz, J.,Gwozdz, T.,Kochman, M.,Jaskolski, M.,Ozyhar, A.
Novel DNA-binding element within the C-terminal extension of the nuclear receptor DNA-binding domain.
Nucleic Acids Res., 35:2705-2718, 2007

PubMed Abstract: The heterodimer of the ecdysone receptor (EcR) and ultraspiracle (Usp), members of the nuclear receptors superfamily, is considered as the functional receptor for ecdysteroids initiating molting and metamorphosis in insects. Here we report the 1.95 A structure of the complex formed by the DNA-binding domains (DBDs) the EcR and the Usp, bound to the natural pseudopalindromic response element. Comparison of the structure with that obtained previously, using an idealized response element, shows how the EcRDBD, which has been previously reported to possess extraordinary flexibility, accommodates DNA-induced structural changes. Part of the C-terminal extension (CTE) of the EcRDBD folds into an alpha-helix whose location in the minor groove does not match any of the locations previously observed for nuclear receptors. Mutational analyses suggest that the alpha-helix is a component of EcR-box, a novel element indispensable for DNA-binding and located within the nuclear receptor CTE. This element seems to be a general feature of all known EcRs.

PubMed: 17426125
DOI: 10.1093/nar/gkm162

Experimental method

X-RAY DIFFRACTION (1.95 Å)