2GX5

N-terminal GAF domain of transcriptional pleiotropic repressor CodY

2GX5 の概要

• エントリーDOI: 10.2210/pdb2gx5/pdb
• 関連するPDBエントリー: 2B0L 2B18
• 分子名称: GTP-sensing transcriptional pleiotropic repressor codY, SULFATE ION, TRIETHYLENE GLYCOL, ... (8 entities in total)
• 機能のキーワード: cody, dna-binding, nucleotide-binding, repressor, transcription regulation, gaf domain, branched chain amino acid binding, transcription
• 由来する生物種: Bacillus subtilis
• 細胞内の位置: Cytoplasm: P39779
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 79941.08

構造登録者

Wilkinson, A.J.,Levdikov, V.M.,Blagova, E.V. (登録日: 2006-05-08, 公開日: 2007-04-17, 最終更新日: 2023-08-30)

主引用文献

Levdikov, V.M.,Blagova, E.,Joseph, P.,Sonenshein, A.L.,Wilkinson, A.J.
The structure of CodY, a GTP- and isoleucine-responsive regulator of stationary phase and virulence in gram-positive bacteria.
J.Biol.Chem., 281:11366-11373, 2006

PubMed Abstract: CodY is a global regulator of transcription in gram-positive bacteria. It represses during growth genes required for adaptation to nutrient limitation, including virulence genes in some human pathogens. CodY activity is regulated by GTP and branched chain amino acids, metabolites whose intracellular concentrations drop as cells enter stationary phase. Although CodY has a highly conserved sequence, it has no significant similarity to proteins of known structure. Here we report crystal structures of two fragments of CodY from Bacillus subtilis that clearly constitute its cofactor and DNA binding domains and reveal that CodY is a chimera of previously observed folding units. The N-terminal cofactor-binding fragment adopts a fold reminiscent of the GAF domains found in cyclic nucleotide phosphodiesterases and adenylate cyclases. It is a dimer stabilized by an intermolecular six alpha-helical bundle that buries an extensive apolar surface rich in residues invariant in CodY orthologues. The branched chain amino acid ligands reside in hydrophobic pockets of each monomer distal to the dimer-forming surface. The structure of the C-terminal DNA binding domain belongs to the winged helix-turn-helix family. The implications of the structure for DNA binding by CodY and its control by cofactor binding are discussed.

PubMed: 16488888
DOI: 10.1074/jbc.M513015200

実験手法

X-RAY DIFFRACTION (1.74 Å)