2GRX

Crystal structure of TonB in complex with FhuA, E. coli outer membrane receptor for ferrichrome

2GRX の概要

• エントリーDOI: 10.2210/pdb2grx/pdb
• 分子名称: Ferrichrome-iron receptor, MYRISTIC ACID, Protein tonB, ... (10 entities in total)
• 機能のキーワード: beta barrel, outer membrane, heterocomplex, inter-protein beta sheet, protein-protein, metal transport
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 216872.03

構造登録者

Pawelek, P.D.,Allaire, M.,Coulton, J.W. (登録日: 2006-04-25, 公開日: 2006-06-13, 最終更新日: 2024-11-13)

主引用文献

Pawelek, P.D.,Croteau, N.,Ng-Thow-Hing, C.,Khursigara, C.M.,Moiseeva, N.,Allaire, M.,Coulton, J.W.
Structure of TonB in complex with FhuA, E. coli outer membrane receptor.
Science, 312:1399-1402, 2006

PubMed Abstract: The cytoplasmic membrane protein TonB spans the periplasm of the Gram-negative bacterial cell envelope, contacts cognate outer membrane receptors, and facilitates siderophore transport. The outer membrane receptor FhuA from Escherichia coli mediates TonB-dependent import of ferrichrome. We report the 3.3 angstrom resolution crystal structure of the TonB carboxyl-terminal domain in complex with FhuA. TonB contacts stabilize FhuA's amino-terminal residues, including those of the consensus Ton box sequence that form an interprotein beta sheet with TonB through strand exchange. The highly conserved TonB residue arginine-166 is oriented to form multiple contacts with the FhuA cork, the globular domain enclosed by the beta barrel.

PubMed: 16741125
DOI: 10.1126/science.1128057

実験手法

X-RAY DIFFRACTION (3.3 Å)