2GQE

Molecular characterization of the Ran binding zinc finger domain

Summary for 2GQE

• Entry DOI: 10.2210/pdb2gqe/pdb
• Related: 1NJ3 1Q5W
• NMR Information: BMRB: 7158
• Descriptor: Nuclear pore complex protein Nup153, ZINC ION (2 entities in total)
• Functional Keywords: zinc finger, ran, nuclear pore, zinc knuckle, nucleoporin, pore, transport, nup153, nup358, ranbp2, transport protein
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus, nuclear pore complex: P49790
• Total number of polymer chains: 1
• Total formula weight: 3557.50

Authors

Higa, M.M.,Alam, S.L.,Sundquist, W.I.,Ullman, K.S. (deposition date: 2006-04-20, release date: 2007-04-10, Last modification date: 2024-05-29)

Primary citation

Higa, M.M.,Alam, S.L.,Sundquist, W.I.,Ullman, K.S.
Molecular Characterization of the Ran-binding Zinc Finger Domain of Nup153.
J.Biol.Chem., 282:17090-17100, 2007

PubMed Abstract: The nuclear pore complex is the gateway for selective traffic between the nucleus and cytoplasm. To learn how building blocks of the pore can create specific docking sites for transport receptors and regulatory factors, we have studied a zinc finger module present in multiple copies within the nuclear pores of higher eukaryotes. All four zinc fingers of human Nup153 were found to bind the small GTPase Ran with dissociation constants ranging between 5 and 40 mum. In addition a fragment of Nup153 encompassing the four tandem zinc fingers was found to bind Ran with similar affinity. NMR structural studies revealed that a representative Nup153 zinc finger adopts the same zinc ribbon structure as the previously characterized Npl4 NZF module. Ran binding was mediated by a three-amino acid motif (Leu(13)/Val(14)/Asn(25)) located within the two zinc coordination loops. Nup153 ZnFs bound GDP and GTP forms of Ran with similar affinities, indicating that this interaction is not influenced by a nucleotide-dependent conformational switch. Taken together, these studies elucidate the Ran-binding interface on Nup153 and, more broadly, provide insight into the versatility of this zinc finger binding module.

PubMed: 17426026
DOI: 10.1074/jbc.M702715200

Experimental method

SOLUTION NMR