2GLG
NMR structure of the [L23,A24]-sCT mutant
Summary for 2GLG
| Entry DOI | 10.2210/pdb2glg/pdb |
| Related | 2GLH |
| Descriptor | Calcitonin-1 (1 entity in total) |
| Functional Keywords | a-helix, hormone-growth factor complex, hormone/growth factor |
| Cellular location | Secreted: P01263 |
| Total number of polymer chains | 1 |
| Total formula weight | 3366.82 |
| Authors | Andreotti, G.,Lopez-Mendez, B.,Amodeo, P.,Morelli, M.A.,Nakamuta, H.,Motta, A. (deposition date: 2006-04-04, release date: 2006-06-20, Last modification date: 2024-11-13) |
| Primary citation | Andreotti, G.,Mendez, B.L.,Amodeo, P.,Morelli, M.A.,Nakamuta, H.,Motta, A. Structural determinants of salmon calcitonin bioactivity: the role of the Leu-based amphipathic alpha-helix. J.Biol.Chem., 281:24193-24203, 2006 Cited by PubMed Abstract: Salmon calcitonin (sCT) forms an amphipathic helix in the region 9-19, with the C-terminal decapeptide interacting with the helix (Amodeo, P., Motta, A., Strazzullo, G., Castiglione Morelli, M. A. (1999) J. Biomol. NMR 13, 161-174). To uncover the structural requirements for the hormone bioactivity, we investigated several sCT analogs. They were designed so as to alter the length of the central helix by removal and/or replacement of flanking residues and by selectively mutating or deleting residues inside the helix. The helix content was assessed by circular dichroism and NMR spectroscopies; the receptor binding affinity in human breast cancer cell line T 47D and the in vivo hypocalcemic activity were also evaluated. In particular, by NMR spectroscopy and molecular dynamics calculations we studied Leu(23),Ala(24)-sCT in which Pro(23) and Arg(24) were replaced by helix inducing residues. Compared with sCT, it assumes a longer amphipathic alpha-helix, with decreased binding affinity and one-fifth of the hypocalcemic activity, therefore supporting the idea of a relationship between a definite helix length and bioactivity. From the analysis of other sCT mutants, we inferred that the correct helix length is located in the 9-19 region and requires long range interactions and the presence of specific regions of residues within the sequence for high binding affinity and hypocalcemic activity. Taken together, the structural and biological data identify well defined structural parameters of the helix for sCT bioactivity. PubMed: 16766525DOI: 10.1074/jbc.M603528200 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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