2GJ7

Crystal Structure of a gE-gI/Fc complex

2GJ7 の概要

• エントリーDOI: 10.2210/pdb2gj7/pdb
• 関連するPDBエントリー: 2GIY
• 分子名称: Ig gamma-1 chain C region, Glycoprotein E, beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: fc receptor, low resolution, immune system-virus-viral protein complex, immune system/virus/viral protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 140059.14

構造登録者

Sprague, E.R.,Wang, C.,Baker, D.,Bjorkman, P.J. (登録日: 2006-03-30, 公開日: 2006-05-30, 最終更新日: 2024-10-16)

主引用文献

Sprague, E.R.,Wang, C.,Baker, D.,Bjorkman, P.J.
Crystal Structure of the HSV-1 Fc Receptor Bound to Fc Reveals a Mechanism for Antibody Bipolar Bridging.
Plos Biol., 4:1-12, 2006

PubMed Abstract: Herpes simplex virus type-1 expresses a heterodimeric Fc receptor, gE-gI, on the surfaces of virions and infected cells that binds the Fc region of host immunoglobulin G and is implicated in the cell-to-cell spread of virus. gE-gI binds immunoglobulin G at the basic pH of the cell surface and releases it at the acidic pH of lysosomes, consistent with a role in facilitating the degradation of antiviral antibodies. Here we identify the C-terminal domain of the gE ectodomain (CgE) as the minimal Fc-binding domain and present a 1.78-angstroms CgE structure. A 5-angstroms gE-gI/Fc crystal structure, which was independently verified by a theoretical prediction method, reveals that CgE binds Fc at the C(H)2-C(H)3 interface, the binding site for several mammalian and bacterial Fc-binding proteins. The structure identifies interface histidines that may confer pH-dependent binding and regions of CgE implicated in cell-to-cell spread of virus. The ternary organization of the gE-gI/Fc complex is compatible with antibody bipolar bridging, which can interfere with the antiviral immune response.

PubMed: 16646632
DOI: 10.1371/journal.pbio.0040148

実験手法

X-RAY DIFFRACTION (5 Å)