2GIT

Human Class I MHC HLA-A2 in complex with the modified HTLV-1 TAX (Y5K-4-[3-Indolyl]-butyric acid) peptide

2GIT の概要

• エントリーDOI: 10.2210/pdb2git/pdb
• 関連するPDBエントリー: 1DUZ 2AV1 2AV7
• 分子名称: HLA class I histocompatibility antigen, A-2 alpha chain, Beta-2-microglobulin, Transcriptional activator TAX, ... (7 entities in total)
• 機能のキーワード: htlv-1 tax peptide, haptenated peptide, lysine-4-(3-indolyl)-butyric acid, mhc class i, hla-a2, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: Q9TQH5; Secreted: P61769
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 90529.47

構造登録者

Borbulevych, O.Y.,Baker, B.M. (登録日: 2006-03-29, 公開日: 2006-10-03, 最終更新日: 2024-11-20)

主引用文献

Gagnon, S.J.,Borbulevych, O.Y.,Davis-Harrison, R.L.,Turner, R.V.,Damirjian, M.,Wojnarowicz, A.,Biddison, W.E.,Baker, B.M.
T Cell Receptor Recognition via Cooperative Conformational Plasticity.
J.Mol.Biol., 363:228-243, 2006

PubMed Abstract: Although T cell receptor cross-reactivity is a fundamental property of the immune system and is implicated in numerous autoimmune pathologies, the molecular mechanisms by which T cell receptors can recognize and respond to diverse ligands are incompletely understood. In the current study we examined the response of the human T cell lymphotropic virus-1 (HTLV-1) Tax-specific T cell receptor (TCR) A6 to a panel of structurally distinct haptens coupled to the Tax 11-19 peptide with a lysine substitution at position 5 (Tax5K, LLFG[K-hapten]PVYV). The A6 TCR could cross-reactively recognize one of these haptenated peptides, Tax-5K-4-(3-Indolyl)-butyric acid (IBA), presented by HLA-A*0201. The crystal structures of Tax5K-IBA/HLA-A2 free and in complex with A6 reveal that binding is mediated by a mechanism of cooperative conformational plasticity involving conformational changes on both sides of the protein-protein interface, including the TCR complementarity determining region (CDR) loops, Valpha/Vbeta domain orientation, and the hapten-modified peptide. Our findings illustrate the complex role that protein dynamics can play in TCR cross-reactivity and highlight that T cell receptor recognition of ligand can be achieved through diverse and complex molecular mechanisms that can occur simultaneously in the interface, not limited to molecular mimicry and CDR loop shifts.

PubMed: 16962135
DOI: 10.1016/j.jmb.2006.08.045

実験手法

X-RAY DIFFRACTION (1.7 Å)