2GIC

Crystal Structure of a vesicular stomatitis virus nucleocapsid-RNA complex

Summary for 2GIC

Entry DOI	10.2210/pdb2gic/pdb
Descriptor	45-MER, Nucleocapsid protein, URANYL (VI) ION (3 entities in total)
Functional Keywords	nucleocapsid, protein-rna complex, negative strand virus, virus-viral protein-rna complex, virus/viral protein/rna
Biological source	Vesicular stomatitis Indiana virus
Cellular location	Virion : P03521
Total number of polymer chains	6
Total formula weight	255102.66
Authors	Green, T.J.,Zhang, X.,Wertz, G.W.,Luo, M. (deposition date: 2006-03-28, release date: 2006-08-22, Last modification date: 2024-02-14)
Primary citation	Green, T.J.,Zhang, X.,Wertz, G.W.,Luo, M. Structure of the vesicular stomatitis virus nucleoprotein-RNA complex unveils how the RNA is sequestered Science, 313:357-360, 2006 Cited by PubMed Abstract: Vesicular stomatitis virus is a negative-stranded RNA virus. Its nucleoprotein (N) binds the viral genomic RNA and is involved in multiple functions including transcription, replication, and assembly. We have determined a 2.9 angstrom structure of a complex containing 10 molecules of the N protein and 90 bases of RNA. The RNA is tightly sequestered in a cavity at the interface between two lobes of the N protein. This serves to protect the RNA in the absence of polynucleotide synthesis. For the RNA to be accessed, some conformational change in the N protein should be necessary. PubMed: 16778022 DOI: 10.1126/science.1126953 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.92 Å)

Structure validation