2GIC

Crystal Structure of a vesicular stomatitis virus nucleocapsid-RNA complex

2GIC の概要

• エントリーDOI: 10.2210/pdb2gic/pdb
• 分子名称: 45-MER, Nucleocapsid protein, URANYL (VI) ION (3 entities in total)
• 機能のキーワード: nucleocapsid, protein-rna complex, negative strand virus, virus-viral protein-rna complex, virus/viral protein/rna
• 由来する生物種: Vesicular stomatitis Indiana virus
• 細胞内の位置: Virion : P03521
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 255102.66

構造登録者

Green, T.J.,Zhang, X.,Wertz, G.W.,Luo, M. (登録日: 2006-03-28, 公開日: 2006-08-22, 最終更新日: 2024-02-14)

主引用文献

Green, T.J.,Zhang, X.,Wertz, G.W.,Luo, M.
Structure of the vesicular stomatitis virus nucleoprotein-RNA complex unveils how the RNA is sequestered
Science, 313:357-360, 2006

PubMed Abstract: Vesicular stomatitis virus is a negative-stranded RNA virus. Its nucleoprotein (N) binds the viral genomic RNA and is involved in multiple functions including transcription, replication, and assembly. We have determined a 2.9 angstrom structure of a complex containing 10 molecules of the N protein and 90 bases of RNA. The RNA is tightly sequestered in a cavity at the interface between two lobes of the N protein. This serves to protect the RNA in the absence of polynucleotide synthesis. For the RNA to be accessed, some conformational change in the N protein should be necessary.

PubMed: 16778022
DOI: 10.1126/science.1126953

実験手法

X-RAY DIFFRACTION (2.92 Å)