2GEE

Crystal Structure of Human Type III Fibronectin Extradomain B and Domain 8

2GEE の概要

• エントリーDOI: 10.2210/pdb2gee/pdb
• 関連するPDBエントリー: 1fnf 2fnb
• 分子名称: hypothetical protein (2 entities in total)
• 機能のキーワード: fibronectin, eiiib, cancer, neovascularization, cell adhesion, protein binding, oncoprotein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22097.48

構造登録者

Bencharit, S.,Cui, C.B.,Siddiqui, A.,Howard-Williams, E.L.,Aukhil, I. (登録日: 2006-03-19, 公開日: 2006-10-17, 最終更新日: 2023-08-30)

主引用文献

Bencharit, S.,Cui, C.B.,Siddiqui, A.,Howard-Williams, E.L.,Sondek, J.,Zuobi-Hasona, K.,Aukhil, I.
Structural insights into fibronectin type III domain-mediated signaling.
J.Mol.Biol., 367:303-309, 2007

PubMed Abstract: The alternatively spliced type III extradomain B (EIIIB) of fibronectin (FN) is expressed only during embryogenesis, wound healing and tumorigenesis. The biological function of this domain is unclear. We describe here the first crystal structure of the interface between alternatively spliced EIIIB and its adjacent FN type III domain 8 (FN B-8). The opened CC' loop of EIIIB, and the rotation and tilt of EIIIB allow good access to the FG loop of FN-8, which is normally hindered by the CC' loop of FN-7. In addition, the AGEGIP sequence of the CC'' loop of EIIIB replaces the NGQQGN sequence of the CC' loop of FN-7. Finally, the CC'' loop of EIIIB forms an acidic groove with FN-8. These structural findings warrant future studies directed at identifying potential binding partners for FN B-8 interface, linking EIIIB to skeletal and cartilaginous development, wound healing, and tumorigenesis, respectively.

PubMed: 17261313
DOI: 10.1016/j.jmb.2006.10.017

実験手法

X-RAY DIFFRACTION (2.01 Å)