2G8V

B. halodurans RNase H catalytic domain E188A mutant in complex with Mg2+ and RNA/DNA hybrid (reaction product)

Summary for 2G8V

• Entry DOI: 10.2210/pdb2g8v/pdb
• Related: 1ZBF 1ZBI 1ZBL 2G8F 2G8H 2G8I
• Descriptor: 5'-R(P*UP*CP*GP*AP*CP*A)-3', 5'-D(*AP*TP*GP*TP*CP*G)-3', Ribonuclease H, ... (6 entities in total)
• Functional Keywords: rnase h, ribonuclease h, rna/dna hybrid, hydrolase-rna-dna complex, hydrolase/rna/dna
• Biological source: Bacillus halodurans
• Cellular location: Cytoplasm (Potential): Q9KEI9
• Total number of polymer chains: 3
• Total formula weight: 20112.59

Authors

Nowotny, M.,Yang, W. (deposition date: 2006-03-03, release date: 2006-04-25, Last modification date: 2023-08-30)

Primary citation

Nowotny, M.,Yang, W.
Stepwise analyses of metal ions in RNase H catalysis from substrate destabilization to product release.
Embo J., 25:1924-1933, 2006

PubMed Abstract: In two-metal catalysis, metal ion A has been proposed to activate the nucleophile and metal ion B to stabilize the transition state. We recently reported crystal structures of RNase H-RNA/DNA substrate complexes obtained at 1.5-2.2 Angstroms. We have now determined and report here structures of reaction intermediate and product complexes of RNase H at 1.65-1.85 Angstroms. The movement of the two metal ions suggests how they may facilitate RNA hydrolysis during the catalytic process. Firstly, metal ion A may assist nucleophilic attack by moving towards metal ion B and bringing the nucleophile close to the scissile phosphate. Secondly, metal ion B transforms from an irregular coordination in the substrate complex to a more regular geometry in the product complex. The exquisite sensitivity of Mg(2+) to the coordination environment likely destabilizes the enzyme-substrate complex and reduces the energy barrier to form product. Lastly, product release probably requires dissociation of metal ion A, which is inhibited by either high concentrations of divalent cations or mutation of an assisting protein residue.

PubMed: 16601679
DOI: 10.1038/sj.emboj.7601076

Experimental method

X-RAY DIFFRACTION (1.85 Å)