2G36

Crystal structure of Tryptophanyl-tRNA synthetase (EC 6.1.1.2) (Tryptophan-tRNA ligase)(TrpRS) (tm0492) from THERMOTOGA MARITIMA at 2.50 A resolution

Summary for 2G36

• Entry DOI: 10.2210/pdb2g36/pdb
• Descriptor: Tryptophanyl-tRNA synthetase, IRON/SULFUR CLUSTER, TRYPTOPHAN, ... (4 entities in total)
• Functional Keywords: tm0492, tryptophanyl-trna synthetase (ec 6.1.1.2) (tryptophan-trna ligase)(trprs), structural genomics, joint center for structural genomics, jcsg, protein structure initiative, psi, ligase
• Biological source: Thermotoga maritima
• Cellular location: Cytoplasm : Q9WYW2
• Total number of polymer chains: 1
• Total formula weight: 39840.05

Authors

Joint Center for Structural Genomics (JCSG) (deposition date: 2006-02-17, release date: 2006-03-28, Last modification date: 2024-04-03)

Primary citation

Han, G.W.,Yang, X.L.,McMullan, D.,Chong, Y.E.,Krishna, S.S.,Rife, C.L.,Weekes, D.,Brittain, S.M.,Abdubek, P.,Ambing, E.,Astakhova, T.,Axelrod, H.L.,Carlton, D.,Caruthers, J.,Chiu, H.J.,Clayton, T.,Duan, L.,Feuerhelm, J.,Grant, J.C.,Grzechnik, S.K.,Jaroszewski, L.,Jin, K.K.,Klock, H.E.,Knuth, M.W.,Kumar, A.,Marciano, D.,Miller, M.D.,Morse, A.T.,Nigoghossian, E.,Okach, L.,Paulsen, J.,Reyes, R.,van den Bedem, H.,White, A.,Wolf, G.,Xu, Q.,Hodgson, K.O.,Wooley, J.,Deacon, A.M.,Godzik, A.,Lesley, S.A.,Elsliger, M.A.,Schimmel, P.,Wilson, I.A.
Structure of a tryptophanyl-tRNA synthetase containing an iron-sulfur cluster.
Acta Crystallogr.,Sect.F, 66:1326-1334, 2010

PubMed Abstract: A novel aminoacyl-tRNA synthetase that contains an iron-sulfur cluster in the tRNA anticodon-binding region and efficiently charges tRNA with tryptophan has been found in Thermotoga maritima. The crystal structure of TmTrpRS (tryptophanyl-tRNA synthetase; TrpRS; EC 6.1.1.2) reveals an iron-sulfur [4Fe-4S] cluster bound to the tRNA anticodon-binding (TAB) domain and an L-tryptophan ligand in the active site. None of the other T. maritima aminoacyl-tRNA synthetases (AARSs) contain this [4Fe-4S] cluster-binding motif (C-x₂₂-C-x₆-C-x₂-C). It is speculated that the iron-sulfur cluster contributes to the stability of TmTrpRS and could play a role in the recognition of the anticodon.

PubMed: 20944229
DOI: 10.1107/S1744309110037619

Experimental method

X-RAY DIFFRACTION (2.5 Å)