2G1P

Structure of E. coli DNA adenine methyltransferase (DAM)

2G1P の概要

• エントリーDOI: 10.2210/pdb2g1p/pdb
• 関連するPDBエントリー: 1FY3 1Q0S 1Q0T 1YFJ 1YFL
• 分子名称: 5'-D(*TP*CP*TP*AP*GP*AP*TP*CP*TP*AP*GP*A)-3', DNA adenine methylase, S-ADENOSYL-L-HOMOCYSTEINE, ... (5 entities in total)
• 機能のキーワード: dam methylation; gatc recognition; base flipping; bacterial virulence factor, transferase-dna complex, transferase/dna
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 72502.97

構造登録者

Horton, J.R.,Liebert, K.,Bekes, M.,Jeltsch, A.,Cheng, X. (登録日: 2006-02-14, 公開日: 2006-11-21, 最終更新日: 2023-08-30)

主引用文献

Horton, J.R.,Liebert, K.,Bekes, M.,Jeltsch, A.,Cheng, X.
Structure and substrate recognition of the Escherichia coli DNA adenine methyltransferase.
J.Mol.Biol., 358:559-570, 2006

PubMed Abstract: The structure of the Escherichia coli Dam DNA-(adenine-N6)-methyltransferase in complex with cognate DNA was determined at 1.89 A resolution in the presence of S-adenosyl-L-homocysteine. DNA recognition and the dynamics of base-flipping were studied by site-directed mutagenesis, DNA methylation kinetics and fluorescence stopped-flow experiments. Our data illustrate the mechanism of coupling of DNA recognition and base-flipping. Contacts to the non-target strand in the second (3') half of the GATC site are established by R124 to the fourth base-pair, and by L122 and P134 to the third base-pair. The aromatic ring of Y119 intercalates into the DNA between the second and third base-pairs, which is essential for base-flipping to occur. Compared to previous published structures of bacteriophage T4 Dam, three major new observations are made in E.coli Dam. (1) The first Gua is recognized by K9, removal of which abrogates the first base-pair recognition. (2) The flipped target Ade binds to the surface of EcoDam in the absence of S-adenosyl-L-methionine, which illustrates a possible intermediate in the base-flipping pathway. (3) The orphaned Thy residue displays structural flexibility by adopting an extrahelical or intrahelical position where it is in contact to N120.

PubMed: 16524590
DOI: 10.1016/j.jmb.2006.02.028

実験手法

X-RAY DIFFRACTION (1.89 Å)