2FNW

Pseudomonas aeruginosa E2Q/H83Q/M109H-azurin RE(PHEN)(CO)3

Summary for 2FNW

• Entry DOI: 10.2210/pdb2fnw/pdb
• Descriptor: Azurin, COPPER (II) ION, (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I), ... (4 entities in total)
• Functional Keywords: blue-copper, electron-transfer, rhenium, infrared spectroscopy, metal binding protein
• Biological source: Pseudomonas aeruginosa
• Cellular location: Periplasm: P00282
• Total number of polymer chains: 2
• Total formula weight: 28943.47

Authors

Gradinaru, C.,Crane, B.R. (deposition date: 2006-01-11, release date: 2006-10-03, Last modification date: 2024-11-06)

Primary citation

Busby, M.,Gradinaru, C.,Crane, B.R.,Di Bilio, A.J.,Matousek, P.,Towrie, M.,Leigh, B.S.,Richards, J.H.,Vlcek, A.,Gray, H.B.
Excited-state dynamics of structurally characterized [ReI(CO)3(phen)(HisX)]+ (X = 83, 109) Pseudomonas aeruginosa azurins in aqueous solution.
J.Am.Chem.Soc., 128:4365-4370, 2006

PubMed Abstract: The triplet metal-to-ligand charge transfer ((3)MLCT) dynamics of two structurally characterized Re(I)(CO)(3)(phen)(HisX)-modified (phen = 1,10-phenanthroline; X = 83, 109) Pseudomonas aeruginosa azurins have been investigated by picosecond time-resolved infrared (TRIR) spectroscopy in aqueous (D(2)O) solution. The (3)MLCT relaxation dynamics exhibited by the two Re(I)-azurins are very different from those of the sensitizer [Re(I)(CO)(3)(phen)(im)](+) (im = imidazole). Whereas the Re(I)(CO)(3) intramolecular vibrational relaxation in Re(I)(CO)(3)(phen)(HisX)Az (4 ps) is similar to that of [Re(I)(CO)(3)(phen)(im)](+) (2 ps), the medium relaxation is much slower ( approximately 250 vs 9.5 ps); the 250-ps relaxation is attributable to reorientation of D(2)O molecules as well as structural reorganization of the rhenium chromophore and nearby polar amino acids in each of the modified proteins.

PubMed: 16569013
DOI: 10.1021/ja057451+

Experimental method

X-RAY DIFFRACTION (1.4 Å)