2FEY
The structure of stem loop IV of Tetrahymena telomerase RNA
Summary for 2FEY
| Entry DOI | 10.2210/pdb2fey/pdb |
| Descriptor | stem-loop IV of Tetrahymena telomerase RNA (1 entity in total) |
| Functional Keywords | telomerase rna, tetrahymena, stem-loop iv, heptaloop, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 13685.10 |
| Authors | Chen, Y.,Fender, J.,Legassie, J.D.,Jarstfer, M.B.,Bryan, T.M.,Varani, G. (deposition date: 2005-12-16, release date: 2006-06-27, Last modification date: 2024-05-29) |
| Primary citation | Chen, Y.,Fender, J.,Legassie, J.D.,Jarstfer, M.B.,Bryan, T.M.,Varani, G. Structure of stem-loop IV of Tetrahymena telomerase RNA. Embo J., 25:3156-3166, 2006 Cited by PubMed Abstract: Conserved domains within the RNA component of telomerase provide the template for reverse transcription, recruit protein components to the holoenzyme and are required for enzymatic activity. Among the functionally essential domains in ciliate telomerase RNA is stem-loop IV, which strongly stimulates telomerase activity and processivity even when provided in trans. The NMR structure of Tetrahymena thermophila stem-loop IV shows a highly structured distal stem-loop linked to a conformationally flexible template-proximal region by a bulge that severely kinks the entire RNA. Through extensive structure-function studies, we identify residues that contribute to both these structural features and to enzymatic activity, with no apparent effect on the binding of TERT protein. We propose that the bending induced by the GA bulge and the flexibility of the template-proximal region allow positioning of the prestructured apical loop during the catalytic cycle. PubMed: 16778765DOI: 10.1038/sj.emboj.7601195 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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