2F9J

3.0 angstrom resolution structure of a Y22M mutant of the spliceosomal protein p14 bound to a region of SF3b155

Summary for 2F9J

• Entry DOI: 10.2210/pdb2f9j/pdb
• Related: 2f9d
• Descriptor: Pre-mRNA branch site protein p14, Splicing factor 3B subunit 1 (3 entities in total)
• Functional Keywords: sf3bp14 sf3b155 sap155 p14y22m rrm, rna binding protein
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus : Q9Y3B4; Nucleus speckle: O75533
• Total number of polymer chains: 4
• Total formula weight: 37973.53

Authors

Schellenberg, M.J.,MacMillan, A.M. (deposition date: 2005-12-06, release date: 2006-01-24, Last modification date: 2023-08-30)

Primary citation

Schellenberg, M.J.,Edwards, R.A.,Ritchie, D.B.,Kent, O.A.,Golas, M.M.,Stark, H.,Glover, J.N.M.,Macmillan, A.M.
Crystal structure of a core spliceosomal protein interface
Proc.Natl.Acad.Sci.Usa, 103:1266-1271, 2006

PubMed Abstract: The precise excision of introns from precursor mRNAs (pre-mRNAs) in eukaryotes is accomplished by the spliceosome, a complex assembly containing five small nuclear ribonucleoprotein (snRNP) particles. Human p14, a component of the spliceosomal U2 and U11/U12 snRNPs, has been shown to associate directly with the pre-mRNA branch adenosine early in spliceosome assembly and within the fully assembled spliceosome. Here we report the 2.5-A crystal structure of a complex containing p14 and a peptide derived from the p14-associated U2 snRNP component SF3b155. p14 contains an RNA recognition motif (RRM), the surface of which is largely occluded by a C-terminal alpha-helix and a portion of the SF3b155 peptide. An analysis of RNA.protein crosslinking to wild-type and mutant p14 shows that the branch adenosine directly interacts with a conserved aromatic within a pocket on the surface of the complex. This result, combined with a comparison of the structure with known RRMs and pseudoRRMs as well as model-building by using the electron cryomicroscopy structure of a spliceosomal U11/U12 di-snRNP, suggests that p14.SF3b155 presents a noncanonical surface for RNA recognition at the heart of the mammalian spliceosome.

PubMed: 16432215
DOI: 10.1073/pnas.0508048103

Experimental method

X-RAY DIFFRACTION (3 Å)