2F69

Ternary complex of SET7/9 bound to AdoHcy and a TAF10 peptide

2F69 の概要

• エントリーDOI: 10.2210/pdb2f69/pdb
• 分子名称: Histone-lysine N-methyltransferase, H3 lysine-4 specific SET7, TAF10 peptide, Acetyl-Ser-Lys-Ser-Mlz-Asp-Arg-Lys-Tyr-Thr-Leu, S-ADENOSYL-L-HOMOCYSTEINE, ... (4 entities in total)
• 機能のキーワード: set domain, protein lysine methyltransferase, enzyme-peptide-adohcy complex, transferase
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: Q8WTS6
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 30696.23

構造登録者

Couture, J.-F.,Collazo, E.,Hauk, G.,Trievel, R.C. (登録日: 2005-11-28, 公開日: 2006-01-17, 最終更新日: 2023-08-23)

主引用文献

Couture, J.-F.,Collazo, E.,Hauk, G.,Trievel, R.C.
Structural basis for the methylation site specificity of SET7/9
Nat.Struct.Mol.Biol., 13:140-146, 2006

PubMed Abstract: Human SET7/9 is a protein lysine methyltransferase (PKMT) that methylates histone H3, the tumor suppressor p53 and the TBP-associated factor TAF10. To elucidate the determinants of its substrate specificity, we have solved the enzyme's structure bound to a TAF10 peptide and examined its ability to methylate histone H3, TAF10 and p53 substrates bearing either mutations or covalent modifications within their respective methylation sites. Collectively, our data reveal that SET7/9 recognizes a conserved K/R-S/T/A motif preceding the lysine substrate and has a propensity to bind aspartates and asparagines on the C-terminal side of the lysine target. We then used a sequence-based approach with this motif to identify novel substrates for this PKMT. Among the putative targets is TAF7, which is methylated at Lys5 by the enzyme in vitro. These results demonstrate the predictive value of the consensus motif in identifying novel substrates for SET7/9.

PubMed: 16415881
DOI: 10.1038/nsmb1045

実験手法

X-RAY DIFFRACTION (1.3 Å)