2F2L

Crystal structure of tracheal cytotoxin (TCT) bound to the ectodomain complex of peptidoglycan recognition proteins LCa (PGRP-LCa) and LCx (PGRP-LCx)

2F2L の概要

• エントリーDOI: 10.2210/pdb2f2l/pdb
• 分子名称: Peptidoglycan-recognition protein-LC isoform LCa, Peptidoglycan recognition protein-LC isoform LCx, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: protein-peptidoglycan complex, membrane protein, immune system, toxin
• 由来する生物種: Drosophila melanogaster (fruit fly); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39538.57

構造登録者

Chang, C.I.,Deisenhofer, J. (登録日: 2005-11-17, 公開日: 2006-04-04, 最終更新日: 2024-11-20)

主引用文献

Chang, C.I.,Chelliah, Y.,Borek, D.,Mengin-Lecreulx, D.,Deisenhofer, J.
Structure of tracheal cytotoxin in complex with a heterodimeric pattern-recognition receptor.
Science, 311:1761-1764, 2006

PubMed Abstract: Tracheal cytotoxin (TCT), a naturally occurring fragment of Gram-negative peptidoglycan, is a potent elicitor of innate immune responses in Drosophila. It induces the heterodimerization of its recognition receptors, the peptidoglycan recognition proteins (PGRPs) LCa and LCx, which activates the immune deficiency pathway. The crystal structure at 2.1 angstrom resolution of TCT in complex with the ectodomains of PGRP-LCa and PGRP-LCx shows that TCT is bound to and presented by the LCx ectodomain for recognition by the LCa ectodomain; the latter lacks a canonical peptidoglycan-docking groove conserved in other PGRPs. The interface, revealed in atomic detail, between TCT and the receptor complex highlights the importance of the anhydro-containing disaccharide in bridging the two ectodomains together and the critical role of diaminopimelic acid as the specificity determinant for PGRP interaction.

PubMed: 16556841
DOI: 10.1126/science.1123056

実験手法

X-RAY DIFFRACTION (2.1 Å)