2F1X

Crystal structure of the TRAF-like domain of HAUSP/USP7 bound to a p53 peptide

2F1X の概要

• エントリーDOI: 10.2210/pdb2f1x/pdb
• 関連するPDBエントリー: 2F1W
• 分子名称: HAUSP/USP7 (2 entities in total)
• 機能のキーワード: traf-like domain, p53 recognition, peptide binding, hausp, usp7, ubp, substrate recognition, hydrolase
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm. Isoform 1: Nucleus. Isoform 2: Nucleus. Isoform 3: Nucleus. Isoform 4: Nucleus. Isoform 7: Nucleus. Isoform 8: Nucleus. Isoform 9: Cytoplasm: P04637
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37413.37

構造登録者

Hu, M.,Gu, L.,Jeffrey, P.D.,Shi, Y. (登録日: 2005-11-15, 公開日: 2006-02-07, 最終更新日: 2023-08-23)

主引用文献

Hu, M.,Gu, L.,Li, M.,Jeffrey, P.D.,Gu, W.,Shi, Y.
Structural Basis of Competitive Recognition of p53 and MDM2 by HAUSP/USP7: Implications for the Regulation of the p53-MDM2 Pathway.
Plos Biol., 4:e27-e27, 2006

PubMed Abstract: Herpesvirus-associated ubiquitin-specific protease (HAUSP, also known as USP7), a deubiquitylating enzyme of the ubiquitin-specific processing protease family, specifically deubiquitylates both p53 and MDM2, hence playing an important yet enigmatic role in the p53-MDM2 pathway. Here we demonstrate that both p53 and MDM2 specifically recognize the N-terminal tumor necrosis factor-receptor associated factor (TRAF)-like domain of HAUSP in a mutually exclusive manner. HAUSP preferentially forms a stable HAUSP-MDM2 complex even in the presence of excess p53. The HAUSP-binding elements were mapped to a peptide fragment in the carboxy-terminus of p53 and to a short-peptide region preceding the acidic domain of MDM2. The crystal structures of the HAUSP TRAF-like domain in complex with p53 and MDM2 peptides, determined at 2.3-A and 1.7-A resolutions, respectively, reveal that the MDM2 peptide recognizes the same surface groove in HAUSP as that recognized by p53 but mediates more extensive interactions. Structural comparison led to the identification of a consensus peptide-recognition sequence by HAUSP. These results, together with the structure of a combined substrate-binding-and-deubiquitylation domain of HAUSP, provide important insights into regulation of the p53-MDM2 pathway by HAUSP.

PubMed: 16402859
DOI: 10.1371/journal.pbio.0040027

実験手法

X-RAY DIFFRACTION (2.3 Å)