2ERM
Solution structure of a biologically active human FGF-1 monomer, complexed to a hexasaccharide heparin-analogue
Summary for 2ERM
| Entry DOI | 10.2210/pdb2erm/pdb |
| Descriptor | Heparin-binding growth factor 1, 2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-acetamido-2-deoxy-6-O-sulfo-alpha-D-glucopyranose-(1-4)-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid, ISOPROPYL ALCOHOL (3 entities in total) |
| Functional Keywords | heparin-like hexasaccharide, fibroblast growth factor, protein-carbohydrate complex, hormone-growth factor complex, hormone/growth factor |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 1 |
| Total formula weight | 17242.99 |
| Authors | Canales, A.,Lozano, R.,Nieto, P.M.,Martin-Lomas, M.,Gimenez-Gallego, G.,Jimenez-Barbero, J. (deposition date: 2005-10-25, release date: 2006-10-03, Last modification date: 2024-05-01) |
| Primary citation | Canales, A.,Lozano, R.,Lopez-Mendez, B.,Angulo, J.,Ojeda, R.,Nieto, P.M.,Martin-Lomas, M.,Gimenez-Gallego, G.,Jimenez-Barbero, J. Solution NMR structure of a human FGF-1 monomer, activated by a hexasaccharide heparin-analogue. Febs J., 273:4716-4727, 2006 Cited by PubMed Abstract: The 3D structure of a complex formed by the acidic fibroblast growth factor (FGF-1) and a specifically designed synthetic heparin hexasaccharide has been determined by NMR spectroscopy. This hexasaccharide can substitute natural heparins in FGF-1 mitogenesis assays, in spite of not inducing any apparent dimerization of the growth factor. The use of this well defined synthetic heparin analogue has allowed us to perform a detailed NMR structural analysis of the heparin-FGF interaction, overcoming the limitations of NMR to deal with the high molecular mass and heterogeneity of the FGF-1 oligomers formed in the presence of natural heparin fragments. Our results confirm that glycosaminoglycans induced FGF-1 dimerization either in a cis or trans disposition with respect to the heparin chain is not an absolute requirement for biological activity. PubMed: 16995857DOI: 10.1111/j.1742-4658.2006.05474.x PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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