2ER8

Crystal Structure of Leu3 DNA-binding domain complexed with a 12mer DNA duplex

2ER8 の概要

• エントリーDOI: 10.2210/pdb2er8/pdb
• 関連するPDBエントリー: 2ERE 2ERG
• 分子名称: 5'-D(*CP*CP*CP*GP*GP*TP*AP*CP*CP*GP*GP*G)-3', Regulatory protein LEU3, ZINC ION, ... (4 entities in total)
• 機能のキーワード: zn(2)cys(6) binuclear cluster motif, transcription activator-dna complex, transcription activator/dna
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Nucleus: P08638
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 49565.00

構造登録者

Fitzgerald, M.X.,Marmorstein, R. (登録日: 2005-10-24, 公開日: 2006-04-04, 最終更新日: 2024-02-14)

主引用文献

Fitzgerald, M.X.,Rojas, J.R.,Kim, J.M.,Kohlhaw, G.B.,Marmorstein, R.
Structure of a Leu3-DNA complex: recognition of everted CGG half-sites by a Zn2Cys6 binuclear cluster protein.
Structure, 14:725-735, 2006

PubMed Abstract: Gal4 is the prototypical Zn2Cys6 binuclear cluster transcriptional regulator that binds as a homodimer to DNA containing inverted CGG half-sites. Leu3, a member of this protein family, binds to everted (opposite polarity to inverted) CGG half-sites, and an H50C mutation within the Leu3 Zn2Cys6 binuclear motif abolishes its transcriptional repression function without impairing DNA binding. We report the X-ray crystal structures of DNA complexes with Leu3 and Leu3(H50C) and solution DNA binding studies of selected Leu3 mutant proteins. These studies reveal the molecular details of everted CGG half-site recognition, and suggest a role for the H50C mutation in transcriptional repression. Comparison with the Gal4-DNA complex shows an unexpected conservation in the DNA recognition mode of inverted and everted CGG half-sites, and points to a critical function of a linker region between the Zn2Cys6 binuclear cluster and dimerization regions in DNA binding specificity. Broader implications of these findings are discussed.

PubMed: 16615914
DOI: 10.1016/j.str.2005.11.025

実験手法

X-RAY DIFFRACTION (2.85 Å)