2EHO

Crystal structure of human GINS complex

2EHO の概要

• エントリーDOI: 10.2210/pdb2eho/pdb
• 分子名称: GINS complex subunit 4, DNA replication complex GINS protein PSF1, DNA replication complex GINS protein PSF2, ... (6 entities in total)
• 機能のキーワード: protein-protein complex, 4-helical bundle, hydrophobic interaction, replication
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm (By similarity): Q9BRT9; Nucleus (By similarity): Q14691 Q9Y248 Q9BRX5
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 270086.14

構造登録者

Choi, J.M.,Lim, H.S.,Kim, J.J.,Song, O.K.,Cho, Y. (登録日: 2007-03-07, 公開日: 2007-06-19, 最終更新日: 2024-10-30)

主引用文献

Choi, J.M.,Lim, H.S.,Kim, J.J.,Song, O.K.,Cho, Y.
Crystal structure of the human GINS complex
Genes Dev., 21:1316-1321, 2007

PubMed Abstract: The GINS complex mediates the assembly of the MCM2-7 (minichromosome maintenance) complex with proteins in a replisome progression complex. The eukaryotic GINS complex is composed of Sld5, Psf1, Psf2, and Psf3, which must be assembled for cell proliferation. We determined the crystal structure of the human GINS complex: GINS forms an elliptical shape with a small central channel. The structures of Sld5 and Psf2 resemble those of Psf1 and Psf3, respectively. In addition, the N-terminal and C-terminal domains of Sld5/Psf1 are permuted in Psf2/Psf3, which suggests that the four proteins have evolved from a common ancestor. Using a structure-based mutational analysis, we identified the functionally critical surface regions of the GINS complex.

PubMed: 17545466
DOI: 10.1101/gad.1548107

実験手法

X-RAY DIFFRACTION (3 Å)