2DJW

Crystal structure of TTHA0845 from Thermus thermophilus HB8

Summary for 2DJW

• Entry DOI: 10.2210/pdb2djw/pdb
• Descriptor: probable transcriptional regulator, AsnC family, ZINC ION (3 entities in total)
• Functional Keywords: structural genomics, thermus thermophilus hb8, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi, unknown function
• Biological source: Thermus thermophilus
• Total number of polymer chains: 10
• Total formula weight: 101477.70

Authors

Okazaki, N.,Nakano, N.,Shinkai, A.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2006-04-05, release date: 2006-09-12, Last modification date: 2024-04-03)

Primary citation

Nakano, N.,Okazaki, N.,Satoh, S.,Takio, K.,Kuramitsu, S.,Shinkai, A.,Yokoyama, S.
Structure of the stand-alone RAM-domain protein from Thermus thermophilus HB8
ACTA CRYSTALLOGR.,SECT.F, 62:855-860, 2006

PubMed Abstract: The stand-alone RAM (regulation of amino-acid metabolism) domain protein SraA from Thermus thermophilus HB8 (TTHA0845) was crystallized in the presence of zinc ions. The X-ray crystal structure was determined using a multiple-wavelength anomalous dispersion technique and was refined at 2.4 A resolution to a final R factor of 25.0%. The monomeric structure is a betaalphabetabetaalphabeta fold and it dimerizes mainly through interactions between the antiparallel beta-sheets. Furthermore, five SraA dimers form a ring with external and internal diameters of 70 and 20 A, respectively. This decameric structure is unique compared with the octameric and dodecameric structures found for other stand-alone RAM-domain proteins and the C-terminal RAM domains of Lrp/AsnC-family proteins.

PubMed: 16946463
DOI: 10.1107/S1744309106031150

Experimental method

X-RAY DIFFRACTION (2.4 Å)