2D9Q

Crystal Structure of the Human GCSF-Receptor Signaling Complex

Summary for 2D9Q

• Entry DOI: 10.2210/pdb2d9q/pdb
• Descriptor: CSF3, Granulocyte colony-stimulating factor receptor, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: cytokine, ligand-receptor complex, signaling protein-cytokine complex, signaling protein/cytokine
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 53819.68

Authors

Tamada, T.,Kuroki, R. (deposition date: 2005-12-12, release date: 2006-02-07, Last modification date: 2023-10-25)

Primary citation

Tamada, T.,Honjo, E.,Maeda, Y.,Okamoto, T.,Ishibashi, M.,Tokunaga, M.,Kuroki, R.
Homodimeric cross-over structure of the human granulocyte colony-stimulating factor (GCSF) receptor signaling complex
Proc.Natl.Acad.Sci.Usa, 103:3135-3140, 2006

PubMed Abstract: A crystal structure of the signaling complex between human granulocyte colony-stimulating factor (GCSF) and a ligand binding region of GCSF receptor (GCSF-R), has been determined to 2.8 A resolution. The GCSF:GCSF-R complex formed a 2:2 stoichiometry by means of a cross-over interaction between the Ig-like domains of GCSF-R and GCSF. The conformation of the complex is quite different from that between human GCSF and the cytokine receptor homologous domain of mouse GCSF-R, but similar to that of the IL-6/gp130 signaling complex. The Ig-like domain cross-over structure necessary for GCSF-R activation is consistent with previously reported thermodynamic and mutational analyses.

PubMed: 16492764
DOI: 10.1073/pnas.0511264103

Experimental method

X-RAY DIFFRACTION (2.8 Å)