2D8W

Structure of HYPER-VIL-trypsin

Summary for 2D8W

• Entry DOI: 10.2210/pdb2d8w/pdb
• Related: 2D8O 2D8P 2D91 2D97 2D98
• Descriptor: Cationic trypsin, CALCIUM ION, IODIDE ION, ... (4 entities in total)
• Functional Keywords: iodo-trypsine, vaporizing iodine labeling(vil), hydrolase
• Biological source: Bos taurus (cattle)
• Cellular location: Secreted, extracellular space: P00760
• Total number of polymer chains: 1
• Total formula weight: 24248.67

Authors

Miyatake, H.,Hasegawa, T.,Yamano, A. (deposition date: 2005-12-08, release date: 2006-07-11, Last modification date: 2024-10-30)

Primary citation

Miyatake, H.,Hasegawa, T.,Yamano, A.
New methods to prepare iodinated derivatives by vaporizing iodine labelling (VIL) and hydrogen peroxide VIL (HYPER-VIL)
ACTA CRYSTALLOGR.,SECT.D, 62:280-289, 2006

PubMed Abstract: New techniques are presented for the preparation of iodine derivatives, involving vapour diffusion of iodine. Firstly, in the vaporizing iodine labelling (VIL) technique, a small amount of KI/I(2) solution is enclosed in a crystallization well, with the result that gaseous I(2) molecules diffuse into the crystallization droplets without exerting substantial changes in ionic strength in the target crystals. Once they have diffused into the droplet, the I(2) molecules sometimes iodinate accessible tyrosines at ortho positions. Secondly, when iodination is insufficient, the hydrogen peroxide VIL (HYPER-VIL) technique can be further applied to increase the iodination ratio by the addition of a small droplet of hydrogen peroxide (H(2)O(2)) to the crystallization well; the gaseous H(2)O(2) also diffuses into the crystallization droplet to emphasize the iodination. These techniques are most effective for phase determination when coupled with softer X-rays, such as those from Cu Kalpha or Cr Kalpha radiation. The effectiveness of these techniques was assessed using five different crystals. Four of the crystals were successfully iodinated, providing sufficient phasing power for structure determination.

PubMed: 16510975
DOI: 10.1107/S0907444905041909

Experimental method

X-RAY DIFFRACTION (2 Å)