2D8W
Structure of HYPER-VIL-trypsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-12 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.323, 58.197, 66.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.310 - 2.000 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.25600 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.811 | 2.130 |
| High resolution limit [Å] | 1.999 | 2.000 |
| Number of reflections | 14772 | |
| Completeness [%] | 99.6 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2.0M ammonium sulfate, 60mM benzamidine, 6mM CaCl2, 50mM Tris/HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
