2CXV

Dual Modes of Modification of Hepatitis A Virus 3C Protease by a Serine-Derived betaLactone: Selective Crystallization and High-resolution Structure of the His-102 Adduct

Summary for 2CXV

• Entry DOI: 10.2210/pdb2cxv/pdb
• Related: 1HAV 1QA7 2A4O
• Descriptor: Probable protein P3C, N-[(BENZYLOXY)CARBONYL]-L-ALANINE (3 entities in total)
• Functional Keywords: hepatitis a, picornavirus, 3c, cysteine protease, inhibitor, beta-lactone, hydrolase
• Biological source: Human hepatitis A virus Hu/Northern Africa/MBB/1978
• Cellular location: Protein VP2: Virion (By similarity). Protein VP3: Virion (By similarity). Protein VP1: Virion (By similarity). Protein VP1-2A: Virion (By similarity). Protein 2B: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 2C: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 3ABC: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 3AB: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 3A: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 3B: Virion (Potential). Picornain 3C: Host cytoplasm (Potential). RNA-directed RNA polymerase 3D-POL: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential): P13901
• Total number of polymer chains: 1
• Total formula weight: 24343.00

Authors

Yin, J.,Bergmann, E.M.,Cherney, M.M.,Lall, M.S.,Jain, R.P.,Vederas, J.C.,James, M.N.G. (deposition date: 2005-07-01, release date: 2005-12-27, Last modification date: 2023-10-25)

• Primary citation: Yin, J.,Bergmann, E.M.,Cherney, M.M.,Lall, M.S.,Jain, R.P.,Vederas, J.C.,James, M.N.G.; Dual Modes of Modification of Hepatitis A Virus 3C Protease by a Serine-derived beta-Lactone: Selective Crystallization and Formation of a Functional Catalytic Triad in the Active Site; J.MOL.BIOL., 354:854-871, 2005; PubMed: 16288920; DOI: 10.1016/j.jmb.2005.09.074

Experimental method

X-RAY DIFFRACTION (1.4 Å)