2CW0

Crystal structure of Thermus thermophilus RNA polymerase holoenzyme at 3.3 angstroms resolution

2CW0 の概要

• エントリーDOI: 10.2210/pdb2cw0/pdb
• 分子名称: DNA-directed RNA polymerase alpha chain, DNA-directed RNA polymerase beta chain, DNA-directed RNA polymerase beta' chain, ... (7 entities in total)
• 機能のキーワード: rna polymerase holoenzyme, transcription, bent-bridge helix, transferase
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 853581.22

構造登録者

Tuske, S.,Sarafianos, S.G.,Wang, X.,Hudson, B.,Sineva, E.,Mukhopadhyay, J.,Birktoft, J.J.,Leroy, O.,Ismail, S.,Clark Jr., A.D.,Dharia, C.,Napoli, A.,Laptenko, O.,Lee, J.,Borukhov, S.,Ebright, R.H.,Arnold, E. (登録日: 2005-06-15, 公開日: 2005-09-20, 最終更新日: 2023-10-25)

主引用文献

Tuske, S.,Sarafianos, S.G.,Wang, X.,Hudson, B.,Sineva, E.,Mukhopadhyay, J.,Birktoft, J.J.,Leroy, O.,Ismail, S.,Clark Jr., A.D.,Dharia, C.,Napoli, A.,Laptenko, O.,Lee, J.,Borukhov, S.,Ebright, R.H.,Arnold, E.
Inhibition of bacterial RNA polymerase by streptolydigin: stabilization of a straight-bridge-helix active-center conformation
Cell(Cambridge,Mass.), 122:541-552, 2005

PubMed Abstract: We define the target, mechanism, and structural basis of inhibition of bacterial RNA polymerase (RNAP) by the tetramic acid antibiotic streptolydigin (Stl). Stl binds to a site adjacent to but not overlapping the RNAP active center and stabilizes an RNAP-active-center conformational state with a straight-bridge helix. The results provide direct support for the proposals that alternative straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations exist and that cycling between straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations is required for RNAP function. The results set bounds on models for RNAP function and suggest strategies for design of novel antibacterial agents.

PubMed: 16122422
DOI: 10.1016/j.cell.2005.07.017

実験手法

X-RAY DIFFRACTION (3.3 Å)