2CH0

Solution structure of the human MAN1 C-terminal domain (residues 655- 775)

Summary for 2CH0

• Entry DOI: 10.2210/pdb2ch0/pdb
• NMR Information: BMRB: 6919
• Descriptor: INNER NUCLEAR MEMBRANE PROTEIN MAN1 (1 entity in total)
• Functional Keywords: man1, winged helix motif, dna, nuclear protein
• Biological source: HOMO SAPIENS (HUMAN)
• Total number of polymer chains: 1
• Total formula weight: 15921.21

Authors

Caputo, S.,Couprie, J.,Duband-Goulet, I.,Lin, F.,Braud, S.,Gondry, M.,Worman, H.J.,Gilquin, B.,Zinn-Justin, S. (deposition date: 2006-03-10, release date: 2006-05-16, Last modification date: 2024-05-15)

Primary citation

Caputo, S.,Couprie, J.,Duband-Goulet, I.,Konde, E.,Lin, F.,Braud, S.,Gondry, M.,Gilquin, B.,Worman, H.J.,Zinn-Justin, S.
The carboxyl-terminal nucleoplasmic region of MAN1 exhibits a DNA binding winged helix domain.
J. Biol. Chem., 281:18208-18215, 2006

PubMed Abstract: MAN1 is an integral protein of the inner nuclear membrane that interacts with nuclear lamins and emerin, thus playing a role in nuclear organization. It also binds to chromatin-associated proteins and transcriptional regulators, including the R-Smads, Smad1, Smad2, and Smad3. Mutations in the human gene encoding MAN1 cause sclerosing bone dysplasias, which sometimes have associated skin abnormalities. At the molecular level, these mutations lead to loss of the MAN1-R-Smads interaction, thus perturbing transforming growth factor beta superfamily signaling pathway. As a first step to understanding the physical basis of MAN1 interaction with R-Smads, we here report the structural characterization of the carboxyl-terminal nucleoplasmic region of MAN1, which is responsible for Smad binding. This region exhibits an amino-terminal globular domain adopting a winged helix fold, as found in several Smad-associated sequence-specific DNA binding factors. Consistently, it binds to DNA through the positively charged recognition helix H3 of its winged helix motif. However, it does not show the predicted carboxyl-terminal U2AF homology domain in solution, suggesting that the folding and stability of such a domain in MAN1 depend upon binding to an unidentified partner. Modeling the complex between DNA and the winged helix domain shows that the regions involved in DNA binding are essentially distinct from those reported to be involved in Smad binding. This suggests that MAN1 binds simultaneously to R-Smads and their targeted DNA sequences.

PubMed: 16648637
DOI: 10.1074/jbc.M601980200

Experimental method

SOLUTION NMR