2CBX

X-ray crystal structure of 5'-fluorodeoxyadenosine synthase from Streptomyces cattleya complexed with beta-D-erythrofuranosyl- adenosine

2CBX の概要

• エントリーDOI: 10.2210/pdb2cbx/pdb
• 関連するPDBエントリー: 1RQP 1RQR 2C2W 2C4T 2C4U 2C5B 2C5H
• 分子名称: 5'-FLUORO-5'-DEOXYADENOSINE SYNTHASE, BETA-D-ERYTHROFURANOSYL-ADENOSINE, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: transferase, fluorinase, 5'-fluorodeoxyadenosine synthase, fla, inhibitor, aza, streptomyces cattleya, sam analogue
• 由来する生物種: STREPTOMYCES CATTLEYA
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 98744.96

構造登録者

McEwan, A.R.,Cadicamo, C.D.,Deng, H.,McGlinchey, R.P.,Robinson, D.R.,O'Hagan, D.,Naismith, J.H.,Spencer, J. (登録日: 2006-01-09, 公開日: 2007-03-06, 最終更新日: 2023-12-13)

主引用文献

Cobb, S.L.,Deng, H.,McEwan, A.R.,Naismith, J.H.,O'Hagan, D.,Robinson, D.A.
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates.
Org. Biomol. Chem., 4:1458-1460, 2006

PubMed Abstract: The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C-F bond. We now report that the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C-F bond formation.

PubMed: 16604208
DOI: 10.1039/b600574h

実験手法

X-RAY DIFFRACTION (2 Å)