2CB3

Crystal structure of peptidoglycan recognition protein-LE in complex with tracheal cytotoxin (monomeric diaminopimelic acid-type peptidoglycan)

2CB3 の概要

• エントリーDOI: 10.2210/pdb2cb3/pdb
• 分子名称: PEPTIDOGLYCAN-RECOGNITION PROTEIN-LE, GLYCEROL, GLCNAC(BETA1-4)-MURNAC(1,6-ANHYDRO)-L-ALA-GAMMA-D-GLU-MESO-A2PM-D-ALA, ... (4 entities in total)
• 機能のキーワード: pgrp, tracheal cytotoxin, innate immunity, immune system
• 由来する生物種: DROSOPHILA MELANOGASTER (FRUIT FLY)
• 細胞内の位置: Secreted: Q9VXN9
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 83922.61

構造登録者

Lim, J.-H.,Kim, M.-S.,Oh, B.-H. (登録日: 2005-12-29, 公開日: 2006-01-26, 最終更新日: 2023-12-13)

主引用文献

Lim, J.-H.,Kim, M.-S.,Kim, H.-E.,Yano, T.,Oshima, Y.,Aggarwal, K.,Goldman, W.E.,Silverman, N.,Kurata, S.,Oh, B.-H.
Structural Basis for Preferential Recognition of Diaminopimelic Acid-Type Peptidoglycan by a Subset of Peptidoglycan Recognition Proteins
J.Biol.Chem., 281:8286-, 2006

PubMed Abstract: Drosophila peptidoglycan recognition protein (PGRP)-LCx and -LCa are receptors that preferentially recognize meso-diaminopimelic acid (DAP)-type peptidoglycan (PGN) present in Gram-negative bacteria over lysine-type PGN of gram-positive bacteria and initiate the IMD signaling pathway, whereas PGRP-LE plays a synergistic role in this process of innate immune defense. How these receptors can distinguish the two types of PGN remains unclear. Here the structure of the PGRP domain of Drosophila PGRP-LE in complex with tracheal cytotoxin (TCT), the monomeric DAP-type PGN, reveals a buried ionic interaction between the unique carboxyl group of DAP and a previously unrecognized arginine residue. This arginine is conserved in the known DAP-type PGN-interacting PGRPs and contributes significantly to the affinity of the protein for the ligand. Unexpectedly, TCT induces infinite head-to-tail dimerization of PGRP-LE, in which the disaccharide moiety, but not the peptide stem, of TCT is positioned at the dimer interface. A sequence comparison suggests that TCT induces heterodimerization of the ectodomains of PGRP-LCx and -LCa in a closely analogous manner to prime the IMD signaling pathway, except that the heterodimer formation is nonperpetuating.

PubMed: 16428381
DOI: 10.1074/JBC.M513030200

実験手法

X-RAY DIFFRACTION (2.4 Å)