2CA6

MIRAS structure determination from hemihedrally twinned crystals

Summary for 2CA6

• Entry DOI: 10.2210/pdb2ca6/pdb
• Related: 1K5D 1K5G 1YRG
• Descriptor: RAN GTPASE-ACTIVATING PROTEIN 1, SULFATE ION (3 entities in total)
• Functional Keywords: gap, gtpase activation, gtpase-activating protein, hemihedral twinning, leucine-rich repeat protein, lrr, merohedral twinning, merohedry, rangap, rna1p, signaling protein, signaling activator, nuclear transport, signaling regulator
• Biological source: SCHIZOSACCHAROMYCES POMBE (FISSION YEAST)
• Total number of polymer chains: 2
• Total formula weight: 86929.56

Authors

Hillig, R.C.,Renault, L. (deposition date: 2005-12-17, release date: 2006-01-11, Last modification date: 2024-05-08)

Primary citation

Hillig, R.C.,Renault, L.
Detecting and Overcoming Hemihedral Twinning During the Mir Structure Determination of RNA1P.
Acta Crystallogr.,Sect.D, 62:750-, 2006

PubMed Abstract: The structure of Rna1p was originally solved to 2.7 A resolution by MIRAS from crystals with partial hemihedral twinning in space group I4(1) [Hillig et al. (1999), Mol. Cell, 3, 781-791] by finding a low-twinned native crystal (twin fraction alpha=0.06) and after twin correction of all data sets. Rna1p crystals have now been used to examine how far twinning and twin correction affect MIR phasing with a higher resolution but highly twinned native data set. Even high hemihedral twinning [alphanative=0.39, alphaderivative=0.24] would not have hindered heavy-atom site identification of strong derivatives using difference Patterson maps. However, a weaker derivative could have been missed and refinement would have stalled at high R values had twinning not been identified and accounted for. Twin correction improved both site identification, experimental phasing statistics and MIR map quality. Different strategies were tested for refinement against twinned data. Using uncorrected twinned data and TWIN-CNS, Rna1p has now been refined to 2.2 A resolution (final twinned R and Rfree were 0.165 and 0.218, respectively). The increased resolution enabled release of the NCS restraints and allowed new conclusions to be drawn on the flexibility of the two molecules in the asymmetric unit. In the case of Rna1p, twinned crystal growth was possible owing to the presence of a twofold NCS axis almost parallel to the twin operator.

PubMed: 16790931
DOI: 10.1107/S0907444906016222

Experimental method

X-RAY DIFFRACTION (2.2 Å)