2C6B

Solution structure of the C4 zinc-finger domain of HDM2

Summary for 2C6B

• Entry DOI: 10.2210/pdb2c6b/pdb
• Related: 1RV1 1T4E 1T4F 1YCR 1Z1M 2C6A
• Descriptor: UBIQUITIN-PROTEIN LIGASE E3 MDM2, ZINC ION (2 entities in total)
• Functional Keywords: zinc finger, human mdm2, ligase, phosphorylation, alternative splicing, metal-binding, nuclear protein, proto- oncogene, ubl conjugation pathway, zinc
• Biological source: HOMO SAPIENS (HUMAN)
• Cellular location: Nucleus, nucleoplasm: Q00987
• Total number of polymer chains: 1
• Total formula weight: 5483.40

Authors

Yu, G.W.,Allen, M.D.,Andreeva, A.,Fersht, A.R.,Bycroft, M. (deposition date: 2005-11-08, release date: 2006-01-04, Last modification date: 2024-05-15)

Primary citation

Yu, G.W.,Allen, M.D.,Andreeva, A.,Fersht, A.R.,Bycroft, M.
Solution Structure of the C4 Zinc Finger Domain of Hdm2.
Protein Sci., 15:384-, 2006

PubMed Abstract: HDM2 is a ubiquitin E3 ligase that is a key negative regulator of the tumor suppressor p53. Here, we report the determination of the solution structure of the C4 zinc finger domain of HDM2 using multidimensional NMR. The HDM2 C4 zinc finger domain has a fold consisting of a 3(10) helix followed by four beta-strands, which shares significant structural similarity to the zinc ribbon protein family. Family based sequence analysis identified two putative binding sites, one of which resembles an RNA binding motif.

PubMed: 16385008
DOI: 10.1110/PS.051927306

Experimental method

SOLUTION NMR