2C1H

The X-ray Structure of Chlorobium vibrioforme 5-Aminolaevulinic Acid Dehydratase Complexed with a Diacid Inhibitor

2C1H の概要

• エントリーDOI: 10.2210/pdb2c1h/pdb
• 関連するPDBエントリー: 1W1Z
• 分子名称: DELTA-AMINOLEVULINIC ACID DEHYDRATASE, MAGNESIUM ION, 4,7-DIOXOSEBACIC ACID, ... (4 entities in total)
• 機能のキーワード: lyase, alad, 5-aminolaevulinic acid dehydratase, heme biosynthesis, magnesium, porphyrin biosynthesis
• 由来する生物種: CHLOROBIUM VIBRIOFORME
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 73378.09

構造登録者

Coates, L.,Beaven, G.,Erskine, P.T.,Beale, S.,Wood, S.P.,Shoolingin-Jordan, P.M.,Cooper, J.B. (登録日: 2005-09-14, 公開日: 2005-12-02, 最終更新日: 2023-12-13)

主引用文献

Coates, L.,Beaven, G.,Erskine, P.T.,Beale, S.,Wood, S.P.,Shoolingin-Jordan, P.M.,Cooper, J.B.
Structure of Chlorobium Vibrioforme 5-Aminolaevulinic Acid Dehydratase Complexed with a Diacid Inhibitor.
Acta Crystallogr.,Sect.D, 61:1594-, 2005

PubMed Abstract: The structure of Chlorobium vibrioforme 5-aminolaevulinic acid dehydratase (ALAD) complexed with the irreversible inhibitor 4,7-dioxosebacic acid has been solved. The inhibitor binds by forming Schiff-base linkages with lysines 200 and 253 at the active site. The structure reported here provides a definition of the interactions made by both of the substrate molecules (A-side and P-side substrates) with the C. vibrioforme ALAD and is compared and contrasted with structures of the same inhibitor bound to Escherichia coli and yeast ALAD. The structure suggests why 4,7-dioxosebacic acid is a better inhibitor of the zinc-dependent ALADs than of the zinc-independent ALADs.

PubMed: 16304458
DOI: 10.1107/S0907444905030350

実験手法

X-RAY DIFFRACTION (2.6 Å)