2BOS

A MUTANT SHIGA-LIKE TOXIN IIE BOUND TO ITS RECEPTOR

Summary for 2BOS

• Entry DOI: 10.2210/pdb2bos/pdb
• Descriptor: PROTEIN (SHIGA-LIKE TOXIN IIE B SUBUNIT), alpha-D-galactopyranose-(1-4)-beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose, alpha-D-galactopyranose-(1-4)-beta-D-galactopyranose, ... (5 entities in total)
• Functional Keywords: toxin, receptor binding, protein-carbohydrate recognition, specificity
• Biological source: Escherichia coli
• Total number of polymer chains: 5
• Total formula weight: 41333.21

Authors

Ling, H.,Boodhoo, A.,Armstrong, G.D.,Clark, C.G.,Brunton, J.L.,Read, R.J. (deposition date: 1998-10-20, release date: 1999-10-20, Last modification date: 2024-10-16)

Primary citation

Ling, H.,Pannu, N.S.,Boodhoo, A.,Armstrong, G.D.,Clark, C.G.,Brunton, J.L.,Read, R.J.
A mutant Shiga-like toxin IIe bound to its receptor Gb(3): structure of a group II Shiga-like toxin with altered binding specificity.
Structure Fold.Des., 8:253-264, 2000

PubMed Abstract: Shiga-like toxins (SLTs) are produced by the pathogenic strains of Escherichia coli that cause hemorrhagic colitis and hemolytic uremic syndrome. These diseases in humans are generally associated with group II family members (SLT-II and SLT-IIc), whereas SLT-IIe (pig edema toxin) is central to edema disease of swine. The pentameric B-subunit component of the majority of family members binds to the cell-surface glycolipid globotriaosyl ceramide (Gb(3)), but globotetraosyl ceramide (Gb(4)) is the preferred receptor for SLT-IIe. A double-mutant of the SLT-IIe B subunit that reverses two sequence differences from SLT-II (GT3; Gln65-->Glu, Lys67-->Gln, SLT-I numbering) has been shown to bind more strongly to Gb(3) than to Gb(4).

PubMed: 10745005
DOI: 10.1016/S0969-2126(00)00103-9

Experimental method

X-RAY DIFFRACTION (2 Å)